Changes in brain gene expression after long-term sleep deprivation Export

@article{citeulike:796174, author = {Cirelli and Chiara and Faraguna and Ugo and Tononi and Giulio}, citeulike-article-id = {796174}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1471-4159.2006.04058.x}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/bsc/jnc/2006/00000098/00000005/art00027}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16923172}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16923172}, comment = {Department of Psychiatry, University of Wisconsin, Madison, Wisconsin, USA. Long-term sleep deprivation in rats produces dramatic physiological changes including increase in energy expenditure, decrease in body weight, and death after 2-3 weeks. Despite several studies, the sleep deprivation syndrome remains largely unexplained. Here, to elucidate how prolonged sleep loss affects brain cells we used microarrays and screened the expression of > 26 000 transcripts in the cerebral cortex. Rats were sleep deprived using the disk-over-water method for 1 week. Seventy-five transcripts showed increased expression in these animals relative to controls that had been spontaneously awake or sleep deprived for a few hours. Most of them were induced as a result of chronic sleep loss and not non-specific effects of the disk stimulation. They include transcripts coding for several immunoglobulins, stress response proteins (macrophage inhibitor factor-related protein 14, heat-shock protein 27, alpha-B-crystallin), minoxidil sulfotransferase, globins and cortistatin. Twenty-eight transcripts decreased their expression in long-term sleep-deprived rats. Sixteen of them were specifically decreased as a result of chronic sleep loss, including those coding for type I procollagen and dihydrolipoamide acetyltransferase. We also compared sleeping rats to short-term and long-term sleep-deprived rats, and found that acute and chronic sleep loss led to some differences at the molecular level. Several plasticity-related genes were strongly induced after acute sleep deprivation only, and several glial genes were down-regulated in both sleep deprivation conditions, but to a different extent. These findings suggest that sustained sleep loss may trigger a generalized inflammatory and stress response in the brain.}, doi = {10.1111/j.1471-4159.2006.04058.x}, issn = {0022-3042}, journal = {Journal of Neurochemistry}, keywords = {chronic, deprivation, expression, gene, microarray, sleep}, month = {September}, number = {5}, pages = {1632--1645}, posted-at = {2006-08-23 20:42:45}, priority = {2}, publisher = {Blackwell Publishing}, title = {Changes in brain gene expression after long-term sleep deprivation}, url = {http://dx.doi.org/10.1111/j.1471-4159.2006.04058.x}, volume = {98}, year = {2006} }

TY - JOUR ID - citeulike:796174 L3 - citeulike-article-id:796174 IS - 5 JF - Journal of Neurochemistry SN - 0022-3042 EP - 1645 TI - Changes in brain gene expression after long-term sleep deprivation PB - Blackwell Publishing VL - 98 SP - 1632 KW - chronic KW - deprivation KW - expression KW - gene KW - microarray KW - sleep N1 - Department of Psychiatry, University of Wisconsin, Madison, Wisconsin, USA. Long-term sleep deprivation in rats produces dramatic physiological changes including increase in energy expenditure, decrease in body weight, and death after 2-3 weeks. Despite several studies, the sleep deprivation syndrome remains largely unexplained. Here, to elucidate how prolonged sleep loss affects brain cells we used microarrays and screened the expression of > 26 000 transcripts in the cerebral cortex. Rats were sleep deprived using the disk-over-water method for 1 week. Seventy-five transcripts showed increased expression in these animals relative to controls that had been spontaneously awake or sleep deprived for a few hours. Most of them were induced as a result of chronic sleep loss and not non-specific effects of the disk stimulation. They include transcripts coding for several immunoglobulins, stress response proteins (macrophage inhibitor factor-related protein 14, heat-shock protein 27, alpha-B-crystallin), minoxidil sulfotransferase, globins and cortistatin. Twenty-eight transcripts decreased their expression in long-term sleep-deprived rats. Sixteen of them were specifically decreased as a result of chronic sleep loss, including those coding for type I procollagen and dihydrolipoamide acetyltransferase. We also compared sleeping rats to short-term and long-term sleep-deprived rats, and found that acute and chronic sleep loss led to some differences at the molecular level. Several plasticity-related genes were strongly induced after acute sleep deprivation only, and several glial genes were down-regulated in both sleep deprivation conditions, but to a different extent. These findings suggest that sustained sleep loss may trigger a generalized inflammatory and stress response in the brain. AU - Cirelli AU - Chiara AU - Faraguna AU - Ugo AU - Tononi AU - Giulio PY - 2006/09// UR - http://dx.doi.org/10.1111/j.1471-4159.2006.04058.x ER -