Measurement of Intracellular Calcium Export

@article{citeulike:1717022, abstract = {Takahashi, Akiyuki, Patricia Camacho, James D. Lechleiter, and Brian Herman. Measurement of Intracellular Calcium. J. Neurophysiol. 79: 1089-1125, 1999. To a certain extent, all cellular, physiological, and pathological phenomena that occur in cells are accompanied by ionic changes. The development of techniques allowing the measurement of such ion activities has contributed substantially to our understanding of normal and abnormal cellular function. Digital video microscopy, confocal laser scanning microscopy, and more recently multiphoton microscopy have allowed the precise spatial analysis of intracellular ion activity at the subcellular level in addition to measurement of its concentration. It is well known that Ca2+ regulates numerous physiological cellular phenomena as a second messenger as well as triggering pathological events such as cell injury and death. A number of methods have been developed to measure intracellular Ca2+. In this review, we summarize the advantages and pitfalls of a variety of Ca2+ indicators used in both optical and nonoptical techniques employed for measuring intracellular Ca2+ concentration.}, author = {Takahashi, Akiyuki and Camacho, Patricia and Lechleiter, James D. and Herman, Brian}, citeulike-article-id = {1717022}, citeulike-linkout-0 = {http://physrev.physiology.org/cgi/content/abstract/79/4/1089}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10508230}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10508230}, day = {1}, journal = {Physiol. Rev.}, keywords = {calcium, dye, fluorescence, measurement, method, review}, month = {October}, number = {4}, pages = {1089--1125}, posted-at = {2008-09-15 10:52:35}, priority = {0}, title = {Measurement of Intracellular Calcium}, url = {http://physrev.physiology.org/cgi/content/abstract/79/4/1089}, volume = {79}, year = {1999} }

TY - JOUR ID - citeulike:1717022 L3 - citeulike-article-id:1717022 N2 - Takahashi, Akiyuki, Patricia Camacho, James D. Lechleiter, and Brian Herman. Measurement of Intracellular Calcium. J. Neurophysiol. 79: 1089-1125, 1999. To a certain extent, all cellular, physiological, and pathological phenomena that occur in cells are accompanied by ionic changes. The development of techniques allowing the measurement of such ion activities has contributed substantially to our understanding of normal and abnormal cellular function. Digital video microscopy, confocal laser scanning microscopy, and more recently multiphoton microscopy have allowed the precise spatial analysis of intracellular ion activity at the subcellular level in addition to measurement of its concentration. It is well known that Ca2+ regulates numerous physiological cellular phenomena as a second messenger as well as triggering pathological events such as cell injury and death. A number of methods have been developed to measure intracellular Ca2+. In this review, we summarize the advantages and pitfalls of a variety of Ca2+ indicators used in both optical and nonoptical techniques employed for measuring intracellular Ca2+ concentration. IS - 4 JF - Physiol. Rev. EP - 1125 TI - Measurement of Intracellular Calcium VL - 79 SP - 1089 KW - calcium KW - dye KW - fluorescence KW - measurement KW - method KW - review AU - Takahashi, Akiyuki AU - Camacho, Patricia AU - Lechleiter, James AU - Herman, Brian PY - 1999/10/01/ UR - http://physrev.physiology.org/cgi/content/abstract/79/4/1089 ER -