Direct evidence for intracellular divalent cation redistribution associated with platelet shape change Export

@article{citeulike:3208230, abstract = {Chlortetracycline was used as a fluorescent probe to monitor shifts in divalent cation distribution when blood platelets were induced to change shape. Human platelets in their native plasma were incubated at 25°C with 50 [mu]M chlortetracycline. It was found that when platelet shape change was stimulated by ADP or the divalent cation ionophore A 23187, a significant decrease in platelet-chlortetracycline fluorescence occurred. This fluorescence shift was consistent with the time course for the change in platelet shape. ATP, which inhibits ADP-induced shape change, also inhibited the decrease in platelet-chlortetracycline fluorescence.}, author = {Le Breton, G. C. and Dinerstein, R. J. and Roth, L. J. and Feinberg, H.}, citeulike-article-id = {3208230}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0006-291X(76)90291-6}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WBK-4DMX883-MH/2/9f86ae3dfc18475d2d2f83f765ae7bf8}, day = {12}, doi = {10.1016/0006-291X(76)90291-6}, journal = {Biochemical and Biophysical Research Communications}, keywords = {calcium, chlortetracycline, fluorescence, measurement, method, platelet}, month = {July}, number = {1}, pages = {362--370}, posted-at = {2008-09-09 08:28:25}, priority = {2}, title = {Direct evidence for intracellular divalent cation redistribution associated with platelet shape change}, url = {http://dx.doi.org/10.1016/0006-291X(76)90291-6}, volume = {71}, year = {1976} }

TY - JOUR ID - citeulike:3208230 L3 - citeulike-article-id:3208230 N2 - Chlortetracycline was used as a fluorescent probe to monitor shifts in divalent cation distribution when blood platelets were induced to change shape. Human platelets in their native plasma were incubated at 25°C with 50 [mu]M chlortetracycline. It was found that when platelet shape change was stimulated by ADP or the divalent cation ionophore A 23187, a significant decrease in platelet-chlortetracycline fluorescence occurred. This fluorescence shift was consistent with the time course for the change in platelet shape. ATP, which inhibits ADP-induced shape change, also inhibited the decrease in platelet-chlortetracycline fluorescence. IS - 1 JF - Biochemical and Biophysical Research Communications EP - 370 TI - Direct evidence for intracellular divalent cation redistribution associated with platelet shape change VL - 71 SP - 362 KW - calcium KW - chlortetracycline KW - fluorescence KW - measurement KW - method KW - platelet AU - Le Breton, GC AU - Dinerstein, RJ AU - Roth, LJ AU - Feinberg, H PY - 1976/07/12/ UR - http://dx.doi.org/10.1016/0006-291X(76)90291-6 ER -