Patch-Clamp Recording of Charge Movement, Ca2+ Current, and Ca2+ Transients in Adult Skeletal Muscle Fibers Export

@article{citeulike:3243216, abstract = {Intramembrane charge movement (Q), Ca2+ conductance (Gm) through the dihydropyridine-sensitive L-type Ca2+ channel (DHPR) and intracellular Ca2+ fluorescence (F) have been recorded simultaneously in flexor digitorum brevis muscle fibers of adult mice, using the whole-cell configuration of the patch-clamp technique. The voltage distribution of Q was fitted to a Boltzmann equation; the Qmax, V1/2Q, and effective valence (zQ) values were 41 {+/-} 3.1 nC/{micro}F, [-]17.6 {+/-} 0.7 mV, and 2.0 {+/-} 0.12, respectively. V1/2G and zG values were [-]0.3 {+/-} 0.06 mV and 5.6 {+/-} 0.34, respectively. Peak Ca2+ transients did not change significantly after 30 min of recording. F was fit to a Boltzmann equation, and the values for VF1/2 and zF were 6.2 {+/-} 0.04 mV and 2.4, respectively. F was adequately fit to the fourth power of Q. These results demonstrate that the patch-clamp technique is appropriate for recording Q, Gm, and intracellular [Ca2+] simultaneously in mature skeletal muscle fibers and that the voltage distribution of the changes in intracellular Ca2+ can be predicted by a Hodgkin-Huxley model.}, author = {Wang, Zhong-Min and Messi, Maria L. and Delbono, Osvaldo}, citeulike-article-id = {3243216}, citeulike-linkout-0 = {http://www.biophysj.org/cgi/content/abstract/77/5/2709}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10545370}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10545370}, day = {1}, journal = {Biophys. J.}, keywords = {5n, calcium, clamp, current, dye, green, method, muscle, patch, skeletal, transient}, month = {November}, number = {5}, pages = {2709--2716}, posted-at = {2008-09-12 03:08:38}, priority = {2}, title = {Patch-Clamp Recording of Charge Movement, Ca2+ Current, and Ca2+ Transients in Adult Skeletal Muscle Fibers}, url = {http://www.biophysj.org/cgi/content/abstract/77/5/2709}, volume = {77}, year = {1999} }

TY - JOUR ID - citeulike:3243216 L3 - citeulike-article-id:3243216 N2 - Intramembrane charge movement (Q), Ca2+ conductance (Gm) through the dihydropyridine-sensitive L-type Ca2+ channel (DHPR) and intracellular Ca2+ fluorescence (F) have been recorded simultaneously in flexor digitorum brevis muscle fibers of adult mice, using the whole-cell configuration of the patch-clamp technique. The voltage distribution of Q was fitted to a Boltzmann equation; the Qmax, V1/2Q, and effective valence (zQ) values were 41 {+/-} 3.1 nC/{micro}F, [-]17.6 {+/-} 0.7 mV, and 2.0 {+/-} 0.12, respectively. V1/2G and zG values were [-]0.3 {+/-} 0.06 mV and 5.6 {+/-} 0.34, respectively. Peak Ca2+ transients did not change significantly after 30 min of recording. F was fit to a Boltzmann equation, and the values for VF1/2 and zF were 6.2 {+/-} 0.04 mV and 2.4, respectively. F was adequately fit to the fourth power of Q. These results demonstrate that the patch-clamp technique is appropriate for recording Q, Gm, and intracellular [Ca2+] simultaneously in mature skeletal muscle fibers and that the voltage distribution of the changes in intracellular Ca2+ can be predicted by a Hodgkin-Huxley model. IS - 5 JF - Biophys. J. EP - 2716 TI - Patch-Clamp Recording of Charge Movement, Ca2+ Current, and Ca2+ Transients in Adult Skeletal Muscle Fibers VL - 77 SP - 2709 KW - 5n KW - calcium KW - clamp KW - current KW - dye KW - green KW - method KW - muscle KW - patch KW - skeletal KW - transient AU - Wang, Zhong-Min AU - Messi, Maria AU - Delbono, Osvaldo PY - 1999/11/01/ UR - http://www.biophysj.org/cgi/content/abstract/77/5/2709 ER -