Measurement of cytosolic free calcium concentration in isolated rat ventricular myocytes with quin 2. Export

@article{citeulike:4364249, abstract = {Cytosolic free calcium concentration was determined in isolated ventricular myocytes from adult rats with the calcium-sensitive indicator, quin 2. The fluorescence signal from resting cells indicated that cytosolic free calcium concentration was 181 +/- 18 nM (mean +/- SEM, n = 18). Inhibition of the sodium-potassium pump with strophanthidin (0.1 mM) resulted in an increase of cytosolic free calcium concentration from 186 +/- 17 to 736 +/- 129 nM (n = 6). The results indicate that it is possible to measure cytosolic free calcium concentration in cardiac muscle cells that have been isolated enzymatically. Moreover, they confirm the observation that inhibition of the sodium-potassium pump increases cytosolic free calcium concentration, presumably via the sodium-calcium exchange mechanism.}, author = {Sheu, S. S. and Sharma, V. K. and Banerjee, S. P.}, citeulike-article-id = {4364249}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/6499138}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=6499138}, issn = {0009-7330}, journal = {Circulation research}, keywords = {calcium, measurement, myocyte, quin-2, rat, ventricle}, month = {December}, number = {6}, pages = {830--834}, posted-at = {2009-04-19 09:13:31}, priority = {2}, title = {Measurement of cytosolic free calcium concentration in isolated rat ventricular myocytes with quin 2.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/6499138}, volume = {55}, year = {1984} }

TY - JOUR ID - citeulike:4364249 L3 - citeulike-article-id:4364249 N2 - Cytosolic free calcium concentration was determined in isolated ventricular myocytes from adult rats with the calcium-sensitive indicator, quin 2. The fluorescence signal from resting cells indicated that cytosolic free calcium concentration was 181 +/- 18 nM (mean +/- SEM, n = 18). Inhibition of the sodium-potassium pump with strophanthidin (0.1 mM) resulted in an increase of cytosolic free calcium concentration from 186 +/- 17 to 736 +/- 129 nM (n = 6). The results indicate that it is possible to measure cytosolic free calcium concentration in cardiac muscle cells that have been isolated enzymatically. Moreover, they confirm the observation that inhibition of the sodium-potassium pump increases cytosolic free calcium concentration, presumably via the sodium-calcium exchange mechanism. IS - 6 JF - Circulation research SN - 0009-7330 EP - 834 TI - Measurement of cytosolic free calcium concentration in isolated rat ventricular myocytes with quin 2. VL - 55 SP - 830 KW - calcium KW - measurement KW - myocyte KW - quin-2 KW - rat KW - ventricle AU - Sheu, SS AU - Sharma, VK AU - Banerjee, SP PY - 1984/12// UR - http://view.ncbi.nlm.nih.gov/pubmed/6499138 ER -