Ca2+ sparks within 200 nm of the sarcolemma of rat ventricular cells: evidence from total internal reflection fluorescence microscopy. Export

@article{citeulike:4365635, abstract = {Total internal reflection fluorescence microscopy (TIRFM) was used to measure local calcium releases in resting cardiac myocytes stained with fluo-3AM. The measured fluorescence originated from regions where cells were close to, and develop adhesions to, a totally reflecting glass surface. The excitation of the fluorescent Ca2+ indicator dye by the exponentially attenuated evanescent wave penetrated approximately 200 nm into the fluid phase. In rat ventricular cells, Ca2+ waves and Ca2+ sparks were observed within the adhesions. Ca2+ sparks recorded with TIRFM compared favorably to sparks recorded under similar conditions with confocal microscopy. Computer simulation supported this assessment. It is concluded that TIRFM can provide an economical, flexible tool for detailed measurement of Ca(2+)-transients in the subsarcolemmal space of live cells.}, author = {Cleemann, L. and DiMassa, G. and Morad, M.}, citeulike-article-id = {4365635}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9330718}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9330718}, issn = {0065-2598}, journal = {Advances in experimental medicine and biology}, keywords = {calcium, cardiac, fluorescence, internal, microscopy, myocyte, reflection, spark, tirf, total, ventricular}, pages = {57--65}, posted-at = {2009-04-19 21:44:14}, priority = {2}, title = {Ca2+ sparks within 200 nm of the sarcolemma of rat ventricular cells: evidence from total internal reflection fluorescence microscopy.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9330718}, volume = {430}, year = {1997} }

TY - JOUR ID - citeulike:4365635 L3 - citeulike-article-id:4365635 N2 - Total internal reflection fluorescence microscopy (TIRFM) was used to measure local calcium releases in resting cardiac myocytes stained with fluo-3AM. The measured fluorescence originated from regions where cells were close to, and develop adhesions to, a totally reflecting glass surface. The excitation of the fluorescent Ca2+ indicator dye by the exponentially attenuated evanescent wave penetrated approximately 200 nm into the fluid phase. In rat ventricular cells, Ca2+ waves and Ca2+ sparks were observed within the adhesions. Ca2+ sparks recorded with TIRFM compared favorably to sparks recorded under similar conditions with confocal microscopy. Computer simulation supported this assessment. It is concluded that TIRFM can provide an economical, flexible tool for detailed measurement of Ca(2+)-transients in the subsarcolemmal space of live cells. JF - Advances in experimental medicine and biology SN - 0065-2598 EP - 65 TI - Ca2+ sparks within 200 nm of the sarcolemma of rat ventricular cells: evidence from total internal reflection fluorescence microscopy. VL - 430 SP - 57 KW - calcium KW - cardiac KW - fluorescence KW - internal KW - microscopy KW - myocyte KW - reflection KW - spark KW - tirf KW - total KW - ventricular AU - Cleemann, L AU - DiMassa, G AU - Morad, M PY - 1997/// UR - http://view.ncbi.nlm.nih.gov/pubmed/9330718 ER -