Low access resistance perforated patch recordings using amphotericin B. Export

@article{citeulike:4402481, abstract = {We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.}, author = {Rae, J. and Cooper, K. and Gates, P. and Watsky, M.}, citeulike-article-id = {4402481}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/2072734}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=2072734}, issn = {0165-0270}, journal = {Journal of neuroscience methods}, keywords = {amphotericin-b, clamp, electrophysiology, method, patch, perforated, technique}, month = {March}, number = {1}, pages = {15--26}, posted-at = {2009-04-25 07:24:47}, priority = {2}, title = {Low access resistance perforated patch recordings using amphotericin B.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2072734}, volume = {37}, year = {1991} }

TY - JOUR ID - citeulike:4402481 L3 - citeulike-article-id:4402481 N2 - We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements. IS - 1 JF - Journal of neuroscience methods SN - 0165-0270 EP - 26 TI - Low access resistance perforated patch recordings using amphotericin B. VL - 37 SP - 15 KW - amphotericin-b KW - clamp KW - electrophysiology KW - method KW - patch KW - perforated KW - technique AU - Rae, J AU - Cooper, K AU - Gates, P AU - Watsky, M PY - 1991/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/2072734 ER -