Occupational genotoxicity risk evaluation through the comet assay and the micronucleus test. Export

@article{citeulike:780208, abstract = {The micronucleus (MN) test and the alkaline single cell gel or comet assay were applied to exfoliated cells of the buccal mucous in order to evaluate the genotoxic risk associated with occupational exposure of 10 storage battery renovation workers, and 10 car painters, with age matched controls, in Pelotas, RS, in southern Brazil. In the MN test, 2000 exfoliated buccal cells were analyzed for each individual, while 100 cells were examined in the comet assay. In the comet test, both comet tail length and a damage index were calculated. Highly significant effects of occupational exposure were found with both the MN test and the comet assay (P<0.001). The comet assay was found to be rapid, of simple visualization, and it is a sensitive technique for measuring and analyzing DNA damage in human cells.}, address = {Escola de Medicina, Universidade Cat\'{o}lica de Pelotas, Pelotas, RS, Brasil. gmartino@brturbo.com}, author = {Martino-Roth, M. G. and Vi\'{e}gas, J. and Roth, D. M.}, citeulike-article-id = {780208}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/15011144}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=15011144}, day = {30}, issn = {1676-5680}, journal = {Genet Mol Res}, keywords = {2003, assay, comet, damage, dna, genotoxicity, medsci302, micronucleus, mutagen, mutagenicity}, month = {December}, number = {4}, pages = {410--417}, posted-at = {2006-07-30 11:15:07}, priority = {3}, title = {Occupational genotoxicity risk evaluation through the comet assay and the micronucleus test.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15011144}, volume = {2}, year = {2003} }

TY - JOUR ID - citeulike:780208 L3 - citeulike-article-id:780208 N2 - The micronucleus (MN) test and the alkaline single cell gel or comet assay were applied to exfoliated cells of the buccal mucous in order to evaluate the genotoxic risk associated with occupational exposure of 10 storage battery renovation workers, and 10 car painters, with age matched controls, in Pelotas, RS, in southern Brazil. In the MN test, 2000 exfoliated buccal cells were analyzed for each individual, while 100 cells were examined in the comet assay. In the comet test, both comet tail length and a damage index were calculated. Highly significant effects of occupational exposure were found with both the MN test and the comet assay (P<0.001). The comet assay was found to be rapid, of simple visualization, and it is a sensitive technique for measuring and analyzing DNA damage in human cells. IS - 4 JF - Genet Mol Res SN - 1676-5680 AD - Escola de Medicina, Universidade Católica de Pelotas, Pelotas, RS, Brasil. gmartino@brturbo.com EP - 417 TI - Occupational genotoxicity risk evaluation through the comet assay and the micronucleus test. VL - 2 SP - 410 KW - 2003 KW - assay KW - comet KW - damage KW - dna KW - genotoxicity KW - medsci302 KW - micronucleus KW - mutagen KW - mutagenicity AU - Martino-Roth, MG AU - Viégas, J AU - Roth, DM PY - 2003/12/30/ UR - http://view.ncbi.nlm.nih.gov/pubmed/15011144 ER -