DSP toxin production de novo in cultures of Dinophysis acuminata (Dinophyceae) from North America. Export

@article{citeulike:4534428, abstract = {For decades, many aspects of Dinophysis biology have remained intractable due to our inability to maintain these organisms in laboratory cultures. Recent breakthroughs in culture methods have opened the door for detailed investigations of these important algae. Here, for the first time, we demonstrate toxin production in cultures of North American Dinophysis acuminata , isolated from Woods Hole, MA. These findings show that, despite the rarity of Dinophysis -related DSP events in North America, D. acuminata from this area has the ability to produce DSP toxins just as it does in other parts of the world where this species is a major cause of DSP toxicity. In our cultures, D. acuminata cells were observed feeding on Myrionecta rubra using a peduncle. Culture extracts were analyzed using LC-MS/MS, providing unequivocal evidence for the toxin DTX1 in the Dinophysis cultures. In addition, a significant amount of an okadaic acid diol ester, OA D8, was detected. These results suggest that this Dinophysis isolate stores much of its OA as a diol ester. Also, toxin PTX-2 and a hydroxylated PTX-2 with identical fragmentation mass spectrum to that of PTX-11, but with a different retention time, were detected in this D. acuminata culture. This demonstration of toxin production in cultured North American Dinophysis sets the stage for more detailed studies investigating the causes of geographic differences in toxicity. It is now clear that North American Dinophysis have the ability to produce DSP toxins even though they only rarely cause toxic DSP events in nature. This may reflect environmental conditions that might induce or repress toxin production, genetic differences that cause modifications in toxin gene expression, or physiological and biochemical differences in prey species.}, author = {Hackett, Jeremiah D. and Tong, Mengmeng and Kulis, David M. and Fux, Elie and Hess, Philipp and Bire, Ronel and Anderson, Donald M.}, citeulike-article-id = {4534428}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.hal.2009.04.004}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1568988309000560}, day = {22}, doi = {10.1016/j.hal.2009.04.004}, issn = {15689883}, journal = {Harmful Algae}, keywords = {acid, diarrhetic, dinophysis, dsp, okadaic, poisioning, shellfish}, month = {April}, posted-at = {2009-06-12 13:05:44}, priority = {2}, title = {DSP toxin production de novo in cultures of Dinophysis acuminata (Dinophyceae) from North America.}, url = {http://dx.doi.org/10.1016/j.hal.2009.04.004}, year = {2009} }

TY - JOUR ID - citeulike:4534428 L3 - citeulike-article-id:4534428 N2 - For decades, many aspects of Dinophysis biology have remained intractable due to our inability to maintain these organisms in laboratory cultures. Recent breakthroughs in culture methods have opened the door for detailed investigations of these important algae. Here, for the first time, we demonstrate toxin production in cultures of North American Dinophysis acuminata , isolated from Woods Hole, MA. These findings show that, despite the rarity of Dinophysis -related DSP events in North America, D. acuminata from this area has the ability to produce DSP toxins just as it does in other parts of the world where this species is a major cause of DSP toxicity. In our cultures, D. acuminata cells were observed feeding on Myrionecta rubra using a peduncle. Culture extracts were analyzed using LC-MS/MS, providing unequivocal evidence for the toxin DTX1 in the Dinophysis cultures. In addition, a significant amount of an okadaic acid diol ester, OA D8, was detected. These results suggest that this Dinophysis isolate stores much of its OA as a diol ester. Also, toxin PTX-2 and a hydroxylated PTX-2 with identical fragmentation mass spectrum to that of PTX-11, but with a different retention time, were detected in this D. acuminata culture. This demonstration of toxin production in cultured North American Dinophysis sets the stage for more detailed studies investigating the causes of geographic differences in toxicity. It is now clear that North American Dinophysis have the ability to produce DSP toxins even though they only rarely cause toxic DSP events in nature. This may reflect environmental conditions that might induce or repress toxin production, genetic differences that cause modifications in toxin gene expression, or physiological and biochemical differences in prey species. JF - Harmful Algae SN - 15689883 TI - DSP toxin production de novo in cultures of Dinophysis acuminata (Dinophyceae) from North America. KW - acid KW - diarrhetic KW - dinophysis KW - dsp KW - okadaic KW - poisioning KW - shellfish AU - Hackett, Jeremiah AU - Tong, Mengmeng AU - Kulis, David AU - Fux, Elie AU - Hess, Philipp AU - Bire, Ronel AU - Anderson, Donald PY - 2009/04/22/ UR - http://dx.doi.org/10.1016/j.hal.2009.04.004 ER -