Lamin B1 controls oxidative stress responses via Oct-1 Export

@article{Malhas_JCB09, abstract = {Interaction of lamins with chromatin and transcription factors regulate transcription. Oct-1 has previously been shown to colocalize partly with B-type lamins and is essential for transcriptional regulation of oxidative stress response genes. Using sequential extraction, co-immunoprecipitation (IP), fluorescence loss in photobleaching, and fluorescence resonance energy transfer, we confirm Oct-1-lamin B1 association at the nuclear periphery and show that this association is lost in Lmnb1{Delta}/{Delta} cells. We show that several Oct-1-dependent genes, including a subset involved in oxidative stress response, are dysregulated in Lmnb1{Delta}/{Delta} cells. Electrophoretic mobility shift assay and chromatin IP reveal that Oct-1 binds to the putative octamer-binding sequences of the dysregulated genes and that this activity is increased in cells lacking functional lamin B1. Like Oct1-/- cells, Lmnb1{Delta}/{Delta} cells have elevated levels of reactive oxygen species and are more susceptible to oxidative stress. Sequestration of Oct-1 at the nuclear periphery by lamin B1 may be a mechanism by which the nuclear envelope can regulate gene expression and contribute to the cellular response to stress, development, and aging. 10.1083/jcb.200804155}, author = {Malhas, Ashraf N. and Lee, Chiu F. and Vaux, David J.}, citeulike-article-id = {5907397}, citeulike-linkout-0 = {http://dx.doi.org/10.1083/jcb.200804155}, citeulike-linkout-1 = {http://jcb.rupress.org/content/184/1/45.abstract}, citeulike-linkout-2 = {http://jcb.rupress.org/content/184/1/45.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19139261}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19139261}, day = {12}, doi = {10.1083/jcb.200804155}, journal = {J. Cell Biol.}, keywords = {chromosome-location, experimental, lamin}, month = {January}, number = {1}, pages = {45--55}, posted-at = {2009-10-07 21:13:00}, priority = {2}, title = {Lamin B1 controls oxidative stress responses via Oct-1}, url = {http://dx.doi.org/10.1083/jcb.200804155}, volume = {184}, year = {2009} }

TY - JOUR ID - Malhas_JCB09 L3 - citeulike-article-id:5907397 N2 - Interaction of lamins with chromatin and transcription factors regulate transcription. Oct-1 has previously been shown to colocalize partly with B-type lamins and is essential for transcriptional regulation of oxidative stress response genes. Using sequential extraction, co-immunoprecipitation (IP), fluorescence loss in photobleaching, and fluorescence resonance energy transfer, we confirm Oct-1-lamin B1 association at the nuclear periphery and show that this association is lost in Lmnb1{Delta}/{Delta} cells. We show that several Oct-1-dependent genes, including a subset involved in oxidative stress response, are dysregulated in Lmnb1{Delta}/{Delta} cells. Electrophoretic mobility shift assay and chromatin IP reveal that Oct-1 binds to the putative octamer-binding sequences of the dysregulated genes and that this activity is increased in cells lacking functional lamin B1. Like Oct1-/- cells, Lmnb1{Delta}/{Delta} cells have elevated levels of reactive oxygen species and are more susceptible to oxidative stress. Sequestration of Oct-1 at the nuclear periphery by lamin B1 may be a mechanism by which the nuclear envelope can regulate gene expression and contribute to the cellular response to stress, development, and aging. 10.1083/jcb.200804155 IS - 1 JF - J. Cell Biol. EP - 55 TI - Lamin B1 controls oxidative stress responses via Oct-1 VL - 184 SP - 45 KW - chromosome-location KW - experimental KW - lamin AU - Malhas, Ashraf AU - Lee, Chiu AU - Vaux, David PY - 2009/01/12/ UR - http://dx.doi.org/10.1083/jcb.200804155 ER -