2D IR provides evidence for mobile water molecules in β-amyloid fibrils Export

@article{Kim_PNAS09, abstract = {10.1073/pnas.0909888106 The motion of water molecules close to amide groups causes their vibrational frequencies to vary rapidly in time. These variations are uniquely sensed by 2-dimensional infrared spectroscopy (2D IR). Here, it is proposed from 2-dimensional experiments on fibrils of amyloid \^{I}² (A\^{I}²)40 that there are water molecules in the fibrils. The spatial locations of the water (DO) were inferred from the responses of 18 amide modes of A\^{I}²40 labeled with C = O. Fast frequency variations were found for residues L17 and V18 and for the apposed residues L34 and V36, suggesting cavities or channels containing mobile water molecules can form between the 2 sheets. Spectroscopic analysis showed that there are 1.2 water molecules per strand in the fibrils. The C = O substitution of 1 residue per strand creates a linear array of isotopologs along the fibril axis that manifests clearly identifiable vibrational transitions. Here, it is shown from the distributions of amide-I\^{a}€² vibrational frequencies that the regularity of these chains is strongly residue dependent and in most cases the distorted regions are also those associated with the putative mobile water molecules. It is proposed that A\^{I}²40 fibrils contain structurally significant mobile water molecules within the intersheet region.}, author = {Kim, Yung S. and Liu, Liu and Axelsen, Paul H. and Hochstrasser, Robin M.}, citeulike-article-id = {5978968}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0909888106}, citeulike-linkout-1 = {http://www.pnas.org/content/106/42/17751.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/106/42/17751.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19815514}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19815514}, day = {20}, doi = {10.1073/pnas.0909888106}, journal = {Proceedings of the National Academy of Sciences}, keywords = {abeta, amyloid, experimental, fibril, infrared\_spectroscopy, structure, water}, month = {October}, number = {42}, pages = {17751--17756}, posted-at = {2009-10-21 09:28:35}, priority = {2}, title = {2D IR provides evidence for mobile water molecules in \^{I}²-amyloid fibrils}, url = {http://dx.doi.org/10.1073/pnas.0909888106}, volume = {106}, year = {2009} }

TY - JOUR ID - Kim_PNAS09 L3 - citeulike-article-id:5978968 N2 - 10.1073/pnas.0909888106 The motion of water molecules close to amide groups causes their vibrational frequencies to vary rapidly in time. These variations are uniquely sensed by 2-dimensional infrared spectroscopy (2D IR). Here, it is proposed from 2-dimensional experiments on fibrils of amyloid β (Aβ)40 that there are water molecules in the fibrils. The spatial locations of the water (DO) were inferred from the responses of 18 amide modes of Aβ40 labeled with C = O. Fast frequency variations were found for residues L17 and V18 and for the apposed residues L34 and V36, suggesting cavities or channels containing mobile water molecules can form between the 2 sheets. Spectroscopic analysis showed that there are 1.2 water molecules per strand in the fibrils. The C = O substitution of 1 residue per strand creates a linear array of isotopologs along the fibril axis that manifests clearly identifiable vibrational transitions. Here, it is shown from the distributions of amide-I′ vibrational frequencies that the regularity of these chains is strongly residue dependent and in most cases the distorted regions are also those associated with the putative mobile water molecules. It is proposed that Aβ40 fibrils contain structurally significant mobile water molecules within the intersheet region. IS - 42 JF - Proceedings of the National Academy of Sciences EP - 17756 TI - 2D IR provides evidence for mobile water molecules in β-amyloid fibrils VL - 106 SP - 17751 KW - abeta KW - amyloid KW - experimental KW - fibril KW - infrared_spectroscopy KW - structure KW - water AU - Kim, Yung AU - Liu, Liu AU - Axelsen, Paul AU - Hochstrasser, Robin PY - 2009/10/20/ UR - http://dx.doi.org/10.1073/pnas.0909888106 ER -