Dynamics of putative raft-associated proteins at the cell surface Export

@article{citeulike:5430136, abstract = {Lipid rafts are conceptualized as membrane microdomains enriched in cholesterol and glycosphingolipid that serve as platforms for protein segregation and signaling. The properties of these domains in vivo are unclear. Here, we use fluorescence recovery after photobleaching to test if raft association affects a protein's ability to laterally diffuse large distances across the cell surface. The diffusion coefficients (D) of several types of putative raft and nonraft proteins were systematically measured under steady-state conditions and in response to raft perturbations. Raft proteins diffused freely over large distances (>4 {micro}m), exhibiting Ds that varied 10-fold. This finding indicates that raft proteins do not undergo long-range diffusion as part of discrete, stable raft domains. Perturbations reported to affect lipid rafts in model membrane systems or by biochemical fractionation (cholesterol depletion, decreased temperature, and cholesterol loading) had similar effects on the diffusional mobility of raft and nonraft proteins. Thus, raft association is not the dominant factor in determining long-range protein mobility at the cell surface. 10.1083/jcb.200312170}, author = {Kenworthy, Anne K. and Nichols, Benjamin J. and Remmert, Catha L. and Hendrix, Glenn M. and Kumar, Mukesh and Zimmerberg, Joshua and Lippincott-Schwartz, Jennifer}, citeulike-article-id = {5430136}, citeulike-linkout-0 = {http://dx.doi.org/10.1083/jcb.200312170}, citeulike-linkout-1 = {http://jcb.rupress.org/cgi/content/abstract/165/5/735}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15173190}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15173190}, day = {7}, doi = {10.1083/jcb.200312170}, journal = {J. Cell Biol.}, keywords = {diffusion, transmembrane}, month = {June}, number = {5}, pages = {735--746}, posted-at = {2009-08-13 03:39:35}, priority = {3}, title = {Dynamics of putative raft-associated proteins at the cell surface}, url = {http://dx.doi.org/10.1083/jcb.200312170}, volume = {165}, year = {2004} }

TY - JOUR ID - citeulike:5430136 L3 - citeulike-article-id:5430136 N2 - Lipid rafts are conceptualized as membrane microdomains enriched in cholesterol and glycosphingolipid that serve as platforms for protein segregation and signaling. The properties of these domains in vivo are unclear. Here, we use fluorescence recovery after photobleaching to test if raft association affects a protein's ability to laterally diffuse large distances across the cell surface. The diffusion coefficients (D) of several types of putative raft and nonraft proteins were systematically measured under steady-state conditions and in response to raft perturbations. Raft proteins diffused freely over large distances (>4 {micro}m), exhibiting Ds that varied 10-fold. This finding indicates that raft proteins do not undergo long-range diffusion as part of discrete, stable raft domains. Perturbations reported to affect lipid rafts in model membrane systems or by biochemical fractionation (cholesterol depletion, decreased temperature, and cholesterol loading) had similar effects on the diffusional mobility of raft and nonraft proteins. Thus, raft association is not the dominant factor in determining long-range protein mobility at the cell surface. 10.1083/jcb.200312170 IS - 5 JF - J. Cell Biol. EP - 746 TI - Dynamics of putative raft-associated proteins at the cell surface VL - 165 SP - 735 KW - diffusion KW - transmembrane AU - Kenworthy, Anne AU - Nichols, Benjamin AU - Remmert, Catha AU - Hendrix, Glenn AU - Kumar, Mukesh AU - Zimmerberg, Joshua AU - Lippincott-Schwartz, Jennifer PY - 2004/06/07/ UR - http://dx.doi.org/10.1083/jcb.200312170 ER -