Description of non-specific DNA-protein interaction and facilitated diffusion with a dynamical model Export

@article{citeulike:3814942, abstract = {We propose a dynamical model for non-specific DNA-protein interaction, which is based on the 'bead-spring' model previously developed by other groups, and investigate its properties using Brownian Dynamics simulations. We show that the model successfully reproduces some of the observed properties of real systems and predictions of kinetic models. For example, sampling of the DNA sequence by the protein proceeds via a succession of 3d motion in the solvent, 1d sliding along the sequence, short hops between neighboring sites, and intersegmental transfers. Moreover, facilitated diffusion takes place in a certain range of values of the protein effective charge, that is, the combination of 1d sliding and 3d motion leads to faster DNA sampling than pure 3d motion. At last, the number of base pairs visited during a sliding event is comparable to the values deduced from single-molecule experiments. We also point out and discuss some discrepancies between the predictions of this model and some recent experimental results as well as some hypotheses and predictions of kinetic models.}, archivePrefix = {arXiv}, author = {Florescu, Ana-Maria and Joyeux, Marc}, citeulike-article-id = {3814942}, citeulike-linkout-0 = {http://arxiv.org/abs/0811.3065}, citeulike-linkout-1 = {http://arxiv.org/pdf/0811.3065}, day = {19}, eprint = {0811.3065}, keywords = {dna-protein, non-specific, th\_ibg}, month = {Nov}, posted-at = {2008-12-20 21:02:44}, priority = {2}, title = {Description of non-specific DNA-protein interaction and facilitated diffusion with a dynamical model}, url = {http://arxiv.org/abs/0811.3065}, year = {2008} }

TY - JOUR ID - citeulike:3814942 L3 - citeulike-article-id:3814942 N2 - We propose a dynamical model for non-specific DNA-protein interaction, which is based on the 'bead-spring' model previously developed by other groups, and investigate its properties using Brownian Dynamics simulations. We show that the model successfully reproduces some of the observed properties of real systems and predictions of kinetic models. For example, sampling of the DNA sequence by the protein proceeds via a succession of 3d motion in the solvent, 1d sliding along the sequence, short hops between neighboring sites, and intersegmental transfers. Moreover, facilitated diffusion takes place in a certain range of values of the protein effective charge, that is, the combination of 1d sliding and 3d motion leads to faster DNA sampling than pure 3d motion. At last, the number of base pairs visited during a sliding event is comparable to the values deduced from single-molecule experiments. We also point out and discuss some discrepancies between the predictions of this model and some recent experimental results as well as some hypotheses and predictions of kinetic models. TI - Description of non-specific DNA-protein interaction and facilitated diffusion with a dynamical model KW - dna-protein KW - non-specific KW - th_ibg AU - Florescu, Ana-Maria AU - Joyeux, Marc PY - 2008/Nov/19/ UR - http://arxiv.org/abs/0811.3065 ER -