Size and composition of membrane protein clusters predicted by Monte Carlo analysis. Export

@article{2004Goldman, abstract = {Biological membranes contain a high density of protein molecules, many of which associate into two-dimensional microdomains with important physiological functions. We have used Monte Carlo simulations to examine the self-association of idealized protein species in two dimensions. The proteins have defined bond strengths and bond angles, allowing us to estimate the size and composition of the aggregates they produce at equilibrium. With a single species of protein, the extent of cluster formation and the sizes of individual clusters both increase in non-linear fashion, showing a "phase change" with protein concentration and bond strength. With multiple co-aggregating proteins, we find that the extent of cluster formation also depends on the relative proportions of participating species. For some lattice geometries, a stoichiometric excess of particular species depresses cluster formation and moreover distorts the composition of clusters that do form. Our results suggest that the self-assembly of microdomains might require a critical level of subunits and that for optimal co-aggregation, proteins should be present in the membrane in the correct stoichiometric ratios.}, address = {Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK.}, author = {Goldman, J. and Andrews, S. and Bray, D.}, citeulike-article-id = {463831}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00249-004-0391-6}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/14997356}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=14997356}, doi = {10.1007/s00249-004-0391-6}, issn = {0175-7571}, journal = {Eur Biophys J}, keywords = {clusters, membrane, protein, refs\_ox}, month = {October}, number = {6}, pages = {506--512}, posted-at = {2006-01-13 03:38:34}, priority = {2}, title = {Size and composition of membrane protein clusters predicted by Monte Carlo analysis.}, url = {http://dx.doi.org/10.1007/s00249-004-0391-6}, volume = {33}, year = {2004} }

TY - JOUR ID - 2004Goldman L3 - citeulike-article-id:463831 N2 - Biological membranes contain a high density of protein molecules, many of which associate into two-dimensional microdomains with important physiological functions. We have used Monte Carlo simulations to examine the self-association of idealized protein species in two dimensions. The proteins have defined bond strengths and bond angles, allowing us to estimate the size and composition of the aggregates they produce at equilibrium. With a single species of protein, the extent of cluster formation and the sizes of individual clusters both increase in non-linear fashion, showing a "phase change" with protein concentration and bond strength. With multiple co-aggregating proteins, we find that the extent of cluster formation also depends on the relative proportions of participating species. For some lattice geometries, a stoichiometric excess of particular species depresses cluster formation and moreover distorts the composition of clusters that do form. Our results suggest that the self-assembly of microdomains might require a critical level of subunits and that for optimal co-aggregation, proteins should be present in the membrane in the correct stoichiometric ratios. IS - 6 JF - Eur Biophys J SN - 0175-7571 AD - Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK. EP - 512 TI - Size and composition of membrane protein clusters predicted by Monte Carlo analysis. VL - 33 SP - 506 KW - clusters KW - membrane KW - protein KW - refs_ox AU - Goldman, J AU - Andrews, S AU - Bray, D PY - 2004/10// UR - http://dx.doi.org/10.1007/s00249-004-0391-6 ER -