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A search for an annual variation of a daily sidereal modulation of the frequency difference between co-located $^129$Xe and $^3$He Zeeman masers sets a stringent limit on boost-dependent Lorentz and CPT violation involving the neutron, consistent with no effect at the level of 150 nHz. In the framework of the general Standard-Model Extension, the present result provides the first clean test for the fermion sector of the symmetry of spacetime under boost transformations at a level of $10^-27$ GeV.
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