Monoallelic yet combinatorial expression of variable exons of the protocadherin-alpha gene cluster in single neurons. Export

@article{citeulike:6089335, abstract = {Diverse protocadherin-alpha genes (Pcdha, also called cadherin-related neuronal receptor or CNR) are expressed in the vertebrate brain. Their genomic organization involves multiple variable exons and a set of constant exons, similar to the immunoglobulin (Ig) and T-cell receptor (TCR) genes. This diversity can be used to distinguish neurons. Using polymorphisms that distinguish the C57BL/6 and MSM mouse strains, we analyzed the allelic expression of the Pcdha gene cluster in individual neurons. Single-cell analysis of Purkinje cells using multiple RT-PCR reactions showed the monoallelic and combinatorial expression of each variable exon in the Pcdha genes. This report is the first description to our knowledge of the allelic expression of a diversified receptor family in the central nervous system. The allelic and combinatorial expression of distinct variable exons of the Pcdha genes is a potential mechanism for specifying neuron identity in the brain.}, author = {Esumi, Shigeyuki and Kakazu, Naoki and Taguchi, Yusuke and Hirayama, Teruyoshi and Sasaki, Ayako and Hirabayashi, Takahiro and Koide, Tsuyoshi and Kitsukawa, Takashi and Hamada, Shun and Yagi, Takeshi}, citeulike-article-id = {6089335}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ng1500}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15640798}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15640798}, doi = {10.1038/ng1500}, issn = {1061-4036}, journal = {Nature genetics}, keywords = {brain, monoaellilsm, neuron, protocadherin-alpha}, month = {February}, number = {2}, pages = {171--176}, posted-at = {2009-11-09 16:37:42}, priority = {2}, title = {Monoallelic yet combinatorial expression of variable exons of the protocadherin-alpha gene cluster in single neurons.}, url = {http://dx.doi.org/10.1038/ng1500}, volume = {37}, year = {2005} }

TY - JOUR ID - citeulike:6089335 L3 - citeulike-article-id:6089335 N2 - Diverse protocadherin-alpha genes (Pcdha, also called cadherin-related neuronal receptor or CNR) are expressed in the vertebrate brain. Their genomic organization involves multiple variable exons and a set of constant exons, similar to the immunoglobulin (Ig) and T-cell receptor (TCR) genes. This diversity can be used to distinguish neurons. Using polymorphisms that distinguish the C57BL/6 and MSM mouse strains, we analyzed the allelic expression of the Pcdha gene cluster in individual neurons. Single-cell analysis of Purkinje cells using multiple RT-PCR reactions showed the monoallelic and combinatorial expression of each variable exon in the Pcdha genes. This report is the first description to our knowledge of the allelic expression of a diversified receptor family in the central nervous system. The allelic and combinatorial expression of distinct variable exons of the Pcdha genes is a potential mechanism for specifying neuron identity in the brain. IS - 2 JF - Nature genetics SN - 1061-4036 EP - 176 TI - Monoallelic yet combinatorial expression of variable exons of the protocadherin-alpha gene cluster in single neurons. VL - 37 SP - 171 KW - brain KW - monoaellilsm KW - neuron KW - protocadherin-alpha AU - Esumi, Shigeyuki AU - Kakazu, Naoki AU - Taguchi, Yusuke AU - Hirayama, Teruyoshi AU - Sasaki, Ayako AU - Hirabayashi, Takahiro AU - Koide, Tsuyoshi AU - Kitsukawa, Takashi AU - Hamada, Shun AU - Yagi, Takeshi PY - 2005/02// UR - http://dx.doi.org/10.1038/ng1500 ER -