Gene expression profiling of lymphoblastoid cell lines from monozygotic twins discordant in severity of autism reveals differential regulation of neurologically relevant genes Export

@article{citeulike:666449, abstract = {Abstract (provisional) Background The autism spectrum encompasses a set of complex multigenic developmental disorders that severely impact the development of language, non-verbal communication, and social skills, and are associated with odd, stereotyped, repetitive behavior and restricted interests. To date, diagnosis of these neurologically based disorders relies predominantly upon behavioral observations often prompted by delayed speech or aberrant behavior, and there are no known genes that can serve as definitive biomarkers for the disorders. Results Here we demonstrate, for the first time, that lymphoblastoid cell lines from monozygotic twins discordant with respect to severity of autism and/or language impairment exhibit differential gene expression patterns on DNA microarrays. Furthermore, we show that genes important to the development, structure, and/or function of the nervous system are among the most differentially expressed genes, and that many of these genes map in silico to chromosomal regions containing previously reported autism candidate genes or quantitative trait loci. Conclusions Our present results provide evidence that candidate genes for autism may be differentially expressed in lymphoid cell lines from individuals with autism spectrum disorders. This finding further suggests the possibility of developing a molecular screen for autism using peripheral lymphocytes, an easily accessible tissue. In addition, gene networks are identified that may play a role in the pathophysiology of autism.}, author = {Hu, Valerie W. and Frank, Bryan C. and Heine, Shannon and Lee, Norman H. and Quackenbush, John}, citeulike-article-id = {666449}, citeulike-linkout-0 = {http://www.biomedcentral.com/1471-2164/7/118/abstract}, citeulike-linkout-1 = {http://dx.doi.org/10.1186/1471-2164-7-118}, doi = {10.1186/1471-2164-7-118}, journal = {BMC Genomics}, keywords = {brain, gene-expression, psychology}, pages = {118}, posted-at = {2006-05-23 20:08:57}, priority = {3}, title = {Gene expression profiling of lymphoblastoid cell lines from monozygotic twins discordant in severity of autism reveals differential regulation of neurologically relevant genes}, url = {http://dx.doi.org/10.1186/1471-2164-7-118}, volume = {7}, year = {2006} }

TY - JOUR ID - citeulike:666449 L3 - citeulike-article-id:666449 N2 - Abstract (provisional) Background The autism spectrum encompasses a set of complex multigenic developmental disorders that severely impact the development of language, non-verbal communication, and social skills, and are associated with odd, stereotyped, repetitive behavior and restricted interests. To date, diagnosis of these neurologically based disorders relies predominantly upon behavioral observations often prompted by delayed speech or aberrant behavior, and there are no known genes that can serve as definitive biomarkers for the disorders. Results Here we demonstrate, for the first time, that lymphoblastoid cell lines from monozygotic twins discordant with respect to severity of autism and/or language impairment exhibit differential gene expression patterns on DNA microarrays. Furthermore, we show that genes important to the development, structure, and/or function of the nervous system are among the most differentially expressed genes, and that many of these genes map in silico to chromosomal regions containing previously reported autism candidate genes or quantitative trait loci. Conclusions Our present results provide evidence that candidate genes for autism may be differentially expressed in lymphoid cell lines from individuals with autism spectrum disorders. This finding further suggests the possibility of developing a molecular screen for autism using peripheral lymphocytes, an easily accessible tissue. In addition, gene networks are identified that may play a role in the pathophysiology of autism. JF - BMC Genomics EP - 118 TI - Gene expression profiling of lymphoblastoid cell lines from monozygotic twins discordant in severity of autism reveals differential regulation of neurologically relevant genes VL - 7 SP - 118 KW - brain KW - gene-expression KW - psychology AU - Hu, Valerie AU - Frank, Bryan AU - Heine, Shannon AU - Lee, Norman AU - Quackenbush, John PY - 2006/// UR - http://dx.doi.org/10.1186/1471-2164-7-118 ER -