Genetic Diversity and Geographic Differentiation in Tacca chantrieri (Taccaceae): an Autonomous Selfing Plant with Showy Floral Display

@article{citeulike:788265, abstract = {* Background and Aims Despite considerable investment in elaborate floral displays, Tacca chantrieri populations are predominantly selfing. It is hypothesized that this species might possess considerable spatial or temporal variation in outcrossing rates among populations. To test this hypothesis, genetic variability and genetic differentiation within and among T. chantrieri populations were investigated to find out if they are in agreement with expectations based on a predominantly inbred mating system. * Methods Genetic diversity was quantified using inter-simple sequence repeats (ISSR) in 303 individuals from 13 populations taken from known locations of T. chantrieri in China, and from one population in Thailand. * Key Results Of the 113 primers screened, 24 produced highly reproducible ISSR bands. Using these primers, 160 discernible DNA fragments were generated, of which 145 (90{middle dot}62 \%) were polymorphic. This indicated considerable genetic variation at the species level. However, there were relatively low levels of polymorphism at population levels, with percentages of polymorphic bands (PPB) ranging from 8{middle dot}75 \% to 55 \%. A high level of genetic differentiation among populations was detected based on different measures (Nei's genetic diversity analysis: GST = 0{middle dot}5835; AMOVA analysis: FST = 0{middle dot}6989). Furthermore, based on levels of genetic differentiation, the 14 populations clustered into two distinct groups separated by the Tanaka Line. * Conclusions High levels of differentiation among populations and low levels of diversity within populations at large spatial scales are consistent with earlier small-scale studies of mating patterns detected by allozymes which showed that T. chantrieri populations are predominantly selfing. However, it appears that T. chantrieri has a mixed-mating system in which self-fertilization predominates, but there is occasional outcrossing. Significant genetic differences between the two distinct regions might be attributed to vicariance along the Tanaka Line. Finally, possible mechanisms of geographic patterns based on genetic differentiation of T. chantrieri are discussed. 10.1093/aob/mcl123}, author = {Zhang, Ling and Li, Qing-Jun and Li, Hong-Tao and Chen, Jin and Li, De-Zhu }, citeulike-article-id = {788265}, doi = {10.1093/aob/mcl123}, journal = {Ann Bot}, keywords = {evolution-selfing\_plant}, month = {August}, number = {2}, pages = {449--457}, posted-at = {2006-08-06 21:29:15}, priority = {2}, title = {Genetic Diversity and Geographic Differentiation in Tacca chantrieri (Taccaceae): an Autonomous Selfing Plant with Showy Floral Display}, url = {http://dx.doi.org/10.1093/aob/mcl123}, volume = {98}, year = {2006} }

TY - JOUR ID - citeulike:788265 L3 - citeulike-article-id:788265 TI - Genetic Diversity and Geographic Differentiation in Tacca chantrieri (Taccaceae): an Autonomous Selfing Plant with Showy Floral Display JF - Ann Bot VL - 98 IS - 2 SP - 449 EP - 457 N2 - * Background and Aims Despite considerable investment in elaborate floral displays, Tacca chantrieri populations are predominantly selfing. It is hypothesized that this species might possess considerable spatial or temporal variation in outcrossing rates among populations. To test this hypothesis, genetic variability and genetic differentiation within and among T. chantrieri populations were investigated to find out if they are in agreement with expectations based on a predominantly inbred mating system. * Methods Genetic diversity was quantified using inter-simple sequence repeats (ISSR) in 303 individuals from 13 populations taken from known locations of T. chantrieri in China, and from one population in Thailand. * Key Results Of the 113 primers screened, 24 produced highly reproducible ISSR bands. Using these primers, 160 discernible DNA fragments were generated, of which 145 (90{middle dot}62 %) were polymorphic. This indicated considerable genetic variation at the species level. However, there were relatively low levels of polymorphism at population levels, with percentages of polymorphic bands (PPB) ranging from 8{middle dot}75 % to 55 %. A high level of genetic differentiation among populations was detected based on different measures (Nei's genetic diversity analysis: GST = 0{middle dot}5835; AMOVA analysis: FST = 0{middle dot}6989). Furthermore, based on levels of genetic differentiation, the 14 populations clustered into two distinct groups separated by the Tanaka Line. * Conclusions High levels of differentiation among populations and low levels of diversity within populations at large spatial scales are consistent with earlier small-scale studies of mating patterns detected by allozymes which showed that T. chantrieri populations are predominantly selfing. However, it appears that T. chantrieri has a mixed-mating system in which self-fertilization predominates, but there is occasional outcrossing. Significant genetic differences between the two distinct regions might be attributed to vicariance along the Tanaka Line. Finally, possible mechanisms of geographic patterns based on genetic differentiation of T. chantrieri are discussed. 10.1093/aob/mcl123 KW - evolution-selfing_plant AU - Zhang, Ling AU - Li, Qing-Jun AU - Li, Hong-Tao AU - Chen, Jin AU - Li, De-Zhu PY - 2006/08/01/ UR - http://dx.doi.org/10.1093/aob/mcl123 ER -