Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos Export

@article{citeulike:1682067, abstract = {Homing endonuclease genes (HEGs) are selfish' genetic elements that combine the capability to selectively disrupt specific gene sequences with the ability to rapidly spread from a few individuals to an entire population through homologous recombination repair events. Because of these properties, HEGs are regarded as promising candidates to transfer genetic modifications from engineered laboratory mosquitoes to wild-type populations including Anopheles gambiae the vector of human malaria. Here we show that I-SceI and I-PpoI homing endonucleases cleave their recognition sites with high efficiency in A. gambiae cells and embryos and we demonstrate HEG-induced homologous and non-homologous repair events in a variety of functional assays. We also propose a gene drive system for mosquitoes that is based on our finding that I-PpoI cuts genomic rDNA located on the X chromosome in A. gambiae, which could be used to selectively incapacitate X-carrying spermatozoa thereby imposing a severe male-biased sex ratio. 10.1093/nar/gkm632}, author = {Windbichler, Nikolai and Papathanos, Philippos A. and Catteruccia, Flaminia and Ranson, Hilary and Burt, Austin and Crisanti, Andrea}, citeulike-article-id = {1682067}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkm632}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17726053}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17726053}, day = {28}, doi = {10.1093/nar/gkm632}, journal = {Nucl. Acids Res.}, keywords = {anopheles, anopheles\_gambiae, endonuclease, gene\_targeting, homing\_endonuclease}, month = {August}, pages = {gkm632+}, posted-at = {2007-09-21 10:38:40}, priority = {2}, title = {Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos}, url = {http://dx.doi.org/10.1093/nar/gkm632}, year = {2007} }

TY - JOUR ID - citeulike:1682067 L3 - citeulike-article-id:1682067 N2 - Homing endonuclease genes (HEGs) are selfish' genetic elements that combine the capability to selectively disrupt specific gene sequences with the ability to rapidly spread from a few individuals to an entire population through homologous recombination repair events. Because of these properties, HEGs are regarded as promising candidates to transfer genetic modifications from engineered laboratory mosquitoes to wild-type populations including Anopheles gambiae the vector of human malaria. Here we show that I-SceI and I-PpoI homing endonucleases cleave their recognition sites with high efficiency in A. gambiae cells and embryos and we demonstrate HEG-induced homologous and non-homologous repair events in a variety of functional assays. We also propose a gene drive system for mosquitoes that is based on our finding that I-PpoI cuts genomic rDNA located on the X chromosome in A. gambiae, which could be used to selectively incapacitate X-carrying spermatozoa thereby imposing a severe male-biased sex ratio. 10.1093/nar/gkm632 JF - Nucl. Acids Res. TI - Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos SP - gkm632 KW - anopheles KW - anopheles_gambiae KW - endonuclease KW - gene_targeting KW - homing_endonuclease AU - Windbichler, Nikolai AU - Papathanos, Philippos AU - Catteruccia, Flaminia AU - Ranson, Hilary AU - Burt, Austin AU - Crisanti, Andrea PY - 2007/08/28/ UR - http://dx.doi.org/10.1093/nar/gkm632 ER -