Development of a sensitive monoclonalantibody-based enzyme-linked immunosorbent assay for the antimalaria active ingredient artemisinin in the Chinese herb Artemisia annua L. Export

@article{citeulike:3778751, abstract = {Abstract\ \ Artemisinin is an endoperoxide sesquiterpene lactone isolated from the Chinese medicinal plant Artemisia annua L. It has been widely used in South-East Asia and Africa as an effective drug against sensitive and multidrug-resistant Plasmodium falciparum malaria. A monoclonal antibody (mAb), designated as 3H2, was generated with artesunate–bovine serum albumin conjugate as the immunogen. mAb 3H2 was used to develop a highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) for artemisinin. The concentration of analyte producing 50\% of inhibition (IC50) and the working range of the icELISA were 1.3 and 0.2–5.8\ ng/mL, respectively. The mAb 3H2 recognized the artemisinin analogs artesunate, dihydroartemisinin, and artemether with cross-reactivity of 650\%, 57\%, and 3\%, respectively, but negligibly recognized deoxyartemisinin and the artemisinin precursors arteannuin B and artemisinic acid. The average recoveries of artemisinin fortified in A. annua samples at concentrations from 156 to 5,000\ μg/g determined by icELISA ranged from 91\% to 98\%. The icELISA was applied for the determination of artemisinin in different wild A. annua samples and the results were confirmed by high-performance liquid chromatography (HPLC) analysis. The correlation coefficient (R 2) between the two assays was larger than 0.99, demonstrating a good agreement between the icELISA and HPLC results. This ELISA is suitable for quality assurance of A. annua L. materials. Figure\  Artemisia annua plant and antimalarial drugs derived from artemisinin}, author = {He, Su-Ping and Tan, Gui-Yu and Li, Gang and Tan, Wei-Ming and Nan, Tie-Gui and Wang, Bao-Min and Li, Zhao-Hu and Li, Qing}, citeulike-article-id = {3778751}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00216-008-2527-5}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19066864}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19066864}, citeulike-linkout-3 = {http://www.springerlink.com/content/780rn35292225768}, doi = {10.1007/s00216-008-2527-5}, journal = {Analytical and Bioanalytical Chemistry}, keywords = {artemisia, artemisinin, assay, elisa, mcab, monoclonal}, posted-at = {2008-12-16 17:01:03}, priority = {2}, title = {Development of a sensitive monoclonalantibody-based enzyme-linked immunosorbent assay for the antimalaria active ingredient artemisinin in the Chinese herb Artemisia annua L.}, url = {http://dx.doi.org/10.1007/s00216-008-2527-5} }

TY - JOUR ID - citeulike:3778751 L3 - citeulike-article-id:3778751 N2 - Abstract  Artemisinin is an endoperoxide sesquiterpene lactone isolated from the Chinese medicinal plant Artemisia annua L. It has been widely used in South-East Asia and Africa as an effective drug against sensitive and multidrug-resistant Plasmodium falciparum malaria. A monoclonal antibody (mAb), designated as 3H2, was generated with artesunate–bovine serum albumin conjugate as the immunogen. mAb 3H2 was used to develop a highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) for artemisinin. The concentration of analyte producing 50% of inhibition (IC50) and the working range of the icELISA were 1.3 and 0.2–5.8 ng/mL, respectively. The mAb 3H2 recognized the artemisinin analogs artesunate, dihydroartemisinin, and artemether with cross-reactivity of 650%, 57%, and 3%, respectively, but negligibly recognized deoxyartemisinin and the artemisinin precursors arteannuin B and artemisinic acid. The average recoveries of artemisinin fortified in A. annua samples at concentrations from 156 to 5,000 μg/g determined by icELISA ranged from 91% to 98%. The icELISA was applied for the determination of artemisinin in different wild A. annua samples and the results were confirmed by high-performance liquid chromatography (HPLC) analysis. The correlation coefficient (R 2) between the two assays was larger than 0.99, demonstrating a good agreement between the icELISA and HPLC results. This ELISA is suitable for quality assurance of A. annua L. materials. Figure  Artemisia annua plant and antimalarial drugs derived from artemisinin JF - Analytical and Bioanalytical Chemistry TI - Development of a sensitive monoclonalantibody-based enzyme-linked immunosorbent assay for the antimalaria active ingredient artemisinin in the Chinese herb Artemisia annua L. KW - artemisia KW - artemisinin KW - assay KW - elisa KW - mcab KW - monoclonal AU - He, Su-Ping AU - Tan, Gui-Yu AU - Li, Gang AU - Tan, Wei-Ming AU - Nan, Tie-Gui AU - Wang, Bao-Min AU - Li, Zhao-Hu AU - Li, Qing UR - http://dx.doi.org/10.1007/s00216-008-2527-5 ER -