An Sau3AI restriction endonuclease isoschizomer from Bacillus cereus Export

@article{citeulike:504337, abstract = {The isolation and characterization of a restriction endonuclease from Bacillus cereus IOC 243 are described. The enzyme recognizes the palindromic sequence 5'-G(met-A,A)TC-3' as determined by PEI chromatography of pancreatic DNase, snake venom phosphodiesterase digestion products of labelled fragments, analysis of restriction digests from normal and N6-methyladenine-free DNA and direct sequence analysis of cloned fragments. The staggered cleavage products with 5'-terminal pGATC extensions are efficiently labelled with polynucleotide kinase and are easily cloned into BamHI sites. The enzyme, denoted Bce243, is thus an isoschizomer of Sau3AI. Its use and potential advantages in substituting Sau3AI are discussed.}, author = {Cruz, A. K. and Kidane, G. and Pires, M. Q. and Rabinovitch, L. and Guaycurus, T. V. and Morel, C. M.}, citeulike-article-id = {504337}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0014-5793(84)81025-X}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6T36-44BMSNB-96/2/a9a684245b11073a37304decba173256}, day = {23}, doi = {10.1016/0014-5793(84)81025-X}, journal = {FEBS Letters}, keywords = {bacillus\_cereus, bce243, cv\_cmmorel, endonuclease, isoschizomer, restriction}, month = {July}, number = {1}, pages = {99--102}, posted-at = {2006-02-13 22:31:46}, priority = {0}, title = {An Sau3AI restriction endonuclease isoschizomer from Bacillus cereus}, url = {http://dx.doi.org/10.1016/0014-5793(84)81025-X}, volume = {173}, year = {1984} }

TY - JOUR ID - citeulike:504337 L3 - citeulike-article-id:504337 N2 - The isolation and characterization of a restriction endonuclease from Bacillus cereus IOC 243 are described. The enzyme recognizes the palindromic sequence 5'-G(met-A,A)TC-3' as determined by PEI chromatography of pancreatic DNase, snake venom phosphodiesterase digestion products of labelled fragments, analysis of restriction digests from normal and N6-methyladenine-free DNA and direct sequence analysis of cloned fragments. The staggered cleavage products with 5'-terminal pGATC extensions are efficiently labelled with polynucleotide kinase and are easily cloned into BamHI sites. The enzyme, denoted Bce243, is thus an isoschizomer of Sau3AI. Its use and potential advantages in substituting Sau3AI are discussed. IS - 1 JF - FEBS Letters EP - 102 TI - An Sau3AI restriction endonuclease isoschizomer from Bacillus cereus VL - 173 SP - 99 KW - bacillus_cereus KW - bce243 KW - cv_cmmorel KW - endonuclease KW - isoschizomer KW - restriction AU - Cruz, AK AU - Kidane, G AU - Pires, MQ AU - Rabinovitch, L AU - Guaycurus, TV AU - Morel, CM PY - 1984/07/23/ UR - http://dx.doi.org/10.1016/0014-5793(84)81025-X ER -