The Listeria transcriptional landscape from saprophytism to virulence. Export

@article{citeulike:4537339, abstract = {The bacterium Listeria monocytogenes is ubiquitous in the environment and can lead to severe food-borne infections. It has recently emerged as a multifaceted model in pathogenesis. However, how this bacterium switches from a saprophyte to a pathogen is largely unknown. Here, using tiling arrays and RNAs from wild-type and mutant bacteria grown in vitro, ex vivo and in vivo, we have analysed the transcription of its entire genome. We provide the complete Listeria operon map and have uncovered far more diverse types of RNAs than expected: in addition to 50 small RNAs (<500 nucleotides), at least two of which are involved in virulence in mice, we have identified antisense RNAs covering several open-reading frames and long overlapping 5' and 3' untranslated regions. We discovered that riboswitches can act as terminators for upstream genes. When Listeria reaches the host intestinal lumen, an extensive transcriptional reshaping occurs with a SigB-mediated activation of virulence genes. In contrast, in the blood, PrfA controls transcription of virulence genes. Remarkably, several non-coding RNAs absent in the non-pathogenic species Listeria innocua exhibit the same expression patterns as the virulence genes. Together, our data unravel successive and coordinated global transcriptional changes during infection and point to previously unknown regulatory mechanisms in bacteria.}, author = {Toledo-Arana, Alejandro and Dussurget, Olivier and Nikitas, Georgios and Sesto, Nina and Guet-Revillet, H\'{e}l\`{e}ne and Balestrino, Damien and Loh, Edmund and Gripenland, Jonas and Tiensuu, Teresa and Vaitkevicius, Karolis and Barthelemy, Mathieu and Vergassola, Massimo and Nahori, Marie-Anne A. and Soubigou, Guillaume and R\'{e}gnault, B\'{e}atrice and Copp\'{e}e, Jean-Yves Y. and Lecuit, Marc and Johansson, J\"{o}rgen and Cossart, Pascale}, citeulike-article-id = {4537339}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature08080}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature08080}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19448609}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19448609}, day = {18}, doi = {10.1038/nature08080}, issn = {1476-4687}, journal = {Nature}, keywords = {tiling\_array}, month = {June}, number = {7249}, pages = {950--956}, posted-at = {2009-06-14 04:18:57}, priority = {2}, publisher = {Nature Publishing Group}, title = {The Listeria transcriptional landscape from saprophytism to virulence.}, url = {http://dx.doi.org/10.1038/nature08080}, volume = {459}, year = {2009} }

TY - JOUR ID - citeulike:4537339 L3 - citeulike-article-id:4537339 N2 - The bacterium Listeria monocytogenes is ubiquitous in the environment and can lead to severe food-borne infections. It has recently emerged as a multifaceted model in pathogenesis. However, how this bacterium switches from a saprophyte to a pathogen is largely unknown. Here, using tiling arrays and RNAs from wild-type and mutant bacteria grown in vitro, ex vivo and in vivo, we have analysed the transcription of its entire genome. We provide the complete Listeria operon map and have uncovered far more diverse types of RNAs than expected: in addition to 50 small RNAs (<500 nucleotides), at least two of which are involved in virulence in mice, we have identified antisense RNAs covering several open-reading frames and long overlapping 5' and 3' untranslated regions. We discovered that riboswitches can act as terminators for upstream genes. When Listeria reaches the host intestinal lumen, an extensive transcriptional reshaping occurs with a SigB-mediated activation of virulence genes. In contrast, in the blood, PrfA controls transcription of virulence genes. Remarkably, several non-coding RNAs absent in the non-pathogenic species Listeria innocua exhibit the same expression patterns as the virulence genes. Together, our data unravel successive and coordinated global transcriptional changes during infection and point to previously unknown regulatory mechanisms in bacteria. IS - 7249 JF - Nature SN - 1476-4687 EP - 956 TI - The Listeria transcriptional landscape from saprophytism to virulence. PB - Nature Publishing Group VL - 459 SP - 950 KW - tiling_array AU - Toledo-Arana, Alejandro AU - Dussurget, Olivier AU - Nikitas, Georgios AU - Sesto, Nina AU - Guet-Revillet, Hélène AU - Balestrino, Damien AU - Loh, Edmund AU - Gripenland, Jonas AU - Tiensuu, Teresa AU - Vaitkevicius, Karolis AU - Barthelemy, Mathieu AU - Vergassola, Massimo AU - Nahori, Marie-Anne AU - Soubigou, Guillaume AU - Régnault, Béatrice AU - Coppée, Jean-Yves AU - Lecuit, Marc AU - Johansson, Jörgen AU - Cossart, Pascale PY - 2009/06/18/ UR - http://dx.doi.org/10.1038/nature08080 ER -