Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli Export

@article{citeulike:3022191, abstract = {Now that many genomes have been sequenced and the products of newly identified genes have been annotated, the next goal is to engineer the desired phenotypes in organisms of interest. For the phenotypic engineering of microorganisms, we have developed novel artificial transcription factors (ATFs) capable of reprogramming innate gene expression circuits in Escherichia coli. These ATFs are composed of zinc finger (ZF) DNA-binding proteins, with distinct specificities, fused to an E. coli cyclic AMP receptor protein (CRP). By randomly assembling 40 different types of ZFs, we have constructed more than 6.4 x 104 ATFs that consist of 3 ZF DNA-binding domains and a CRP effector domain. Using these ATFs, we induced various phenotypic changes in E. coli and selected for industrially important traits, such as resistance to heat shock, osmotic pressure and cold shock. Genes associated with the heat-shock resistance phenotype were then characterized. These results and the general applicability of this platform clearly indicate that novel ATFs are powerful tools for the phenotypic engineering of microorganisms and can facilitate microbial functional genomic studies. 10.1093/nar/gkn449}, author = {Lee, Ju Y. and Sung, Bong H. and Yu, Byung J. and Lee, Jun H. and Lee, Sang H. and Kim, Mi S. and Koob, Michael D. and Kim, Sun C.}, citeulike-article-id = {3022191}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkn449}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18641039}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18641039}, day = {18}, doi = {10.1093/nar/gkn449}, journal = {Nucl. Acids Res.}, keywords = {synthetic-biology}, month = {July}, pages = {gkn449+}, posted-at = {2008-07-20 08:20:35}, priority = {3}, title = {Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli}, url = {http://dx.doi.org/10.1093/nar/gkn449}, year = {2008} }

TY - JOUR ID - citeulike:3022191 L3 - citeulike-article-id:3022191 N2 - Now that many genomes have been sequenced and the products of newly identified genes have been annotated, the next goal is to engineer the desired phenotypes in organisms of interest. For the phenotypic engineering of microorganisms, we have developed novel artificial transcription factors (ATFs) capable of reprogramming innate gene expression circuits in Escherichia coli. These ATFs are composed of zinc finger (ZF) DNA-binding proteins, with distinct specificities, fused to an E. coli cyclic AMP receptor protein (CRP). By randomly assembling 40 different types of ZFs, we have constructed more than 6.4 x 104 ATFs that consist of 3 ZF DNA-binding domains and a CRP effector domain. Using these ATFs, we induced various phenotypic changes in E. coli and selected for industrially important traits, such as resistance to heat shock, osmotic pressure and cold shock. Genes associated with the heat-shock resistance phenotype were then characterized. These results and the general applicability of this platform clearly indicate that novel ATFs are powerful tools for the phenotypic engineering of microorganisms and can facilitate microbial functional genomic studies. 10.1093/nar/gkn449 JF - Nucl. Acids Res. TI - Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli SP - gkn449 KW - synthetic-biology AU - Lee, Ju AU - Sung, Bong AU - Yu, Byung AU - Lee, Jun AU - Lee, Sang AU - Kim, Mi AU - Koob, Michael AU - Kim, Sun PY - 2008/07/18/ UR - http://dx.doi.org/10.1093/nar/gkn449 ER -