Analysing immune cell migration Export

@article{citeulike:6005291, abstract = {The visualization of the dynamic behaviour of and interactions between immune cells using time-lapse video microscopy has an important role in modern immunology. To draw robust conclusions, quantification of such cell migration is required. However, imaging experiments are associated with various artefacts that can affect the estimated positions of the immune cells under analysis, which form the basis of any subsequent analysis. Here, we describe potential artefacts that could affect the interpretation of data sets on immune cell migration. We propose how these errors can be recognized and corrected, and suggest ways to prevent the data analysis itself leading to biased results.}, author = {Beltman, Joost B. and Maree, Athanasius F. M. and de Boer, Rob J.}, citeulike-article-id = {6005291}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nri2638}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nri2638}, day = {16}, doi = {10.1038/nri2638}, issn = {1474-1733}, journal = {Nature Reviews Immunology}, keywords = {migration}, month = {October}, number = {11}, pages = {789--798}, posted-at = {2009-11-06 22:53:45}, priority = {2}, publisher = {Nature Publishing Group}, title = {Analysing immune cell migration}, url = {http://dx.doi.org/10.1038/nri2638}, volume = {9}, year = {2009} }

TY - JOUR ID - citeulike:6005291 L3 - citeulike-article-id:6005291 N2 - The visualization of the dynamic behaviour of and interactions between immune cells using time-lapse video microscopy has an important role in modern immunology. To draw robust conclusions, quantification of such cell migration is required. However, imaging experiments are associated with various artefacts that can affect the estimated positions of the immune cells under analysis, which form the basis of any subsequent analysis. Here, we describe potential artefacts that could affect the interpretation of data sets on immune cell migration. We propose how these errors can be recognized and corrected, and suggest ways to prevent the data analysis itself leading to biased results. IS - 11 JF - Nature Reviews Immunology SN - 1474-1733 EP - 798 TI - Analysing immune cell migration PB - Nature Publishing Group VL - 9 SP - 789 KW - migration AU - Beltman, Joost AU - Maree, Athanasius AU - de Boer, Rob PY - 2009/10/16/ UR - http://dx.doi.org/10.1038/nri2638 ER -