Tissue engineering of tooth crown, root, and periodontium. Export

@article{citeulike:5649943, abstract = {Tissue engineering of teeth requires the coordinated formation of correctly shaped crowns, roots, and periodontal ligament. Previous studies have shown that the dental mesenchyme controls crown morphogenesis and epithelial histogenesis during tooth development in vivo, but little is known about the inductive potential of dissociated mesenchymal cells used in ex vivo cultures. A 2-step method is described in which, by using different types of reassociations between epithelial and mesenchymal tissues and/or cells from mouse embryos, reassociations were cultured in vitro before in vivo implantation. In vitro, the reassociated tissues developed and resulted in tooth-like structures that exhibited normal epithelial histogenesis and allowed the functional differentiation of odontoblasts and ameloblasts. After implantation, the reassociations formed roots and periodontal ligament, the latter connected to developing bone. The shape of the crown, initially suspected to depend on the integrity of the mesenchyme, could be modulated by adjusting the number of dissociated mesenchymal cells reassociated with the epithelial compartment. Based on these results, we propose a refined strategy for tooth tissue engineering that may help to eventually generate morphologically defined teeth.}, author = {Hu, Bing and Nadiri, Amal and Kuchler-Bopp, Sabine and Perrin-Schmitt, Fabienne and Peters, Heiko and Lesot, Herv\'{e}}, citeulike-article-id = {5649943}, citeulike-linkout-0 = {http://dx.doi.org/10.1089/ten.2006.12.2069}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16968149}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16968149}, doi = {10.1089/ten.2006.12.2069}, issn = {1076-3279}, journal = {Tissue engineering}, keywords = {bioengineered\_tooth, crown, periodontium, root}, month = {August}, number = {8}, pages = {2069--2075}, posted-at = {2009-08-26 11:15:10}, priority = {3}, title = {Tissue engineering of tooth crown, root, and periodontium.}, url = {http://dx.doi.org/10.1089/ten.2006.12.2069}, volume = {12}, year = {2006} }

TY - JOUR ID - citeulike:5649943 L3 - citeulike-article-id:5649943 N2 - Tissue engineering of teeth requires the coordinated formation of correctly shaped crowns, roots, and periodontal ligament. Previous studies have shown that the dental mesenchyme controls crown morphogenesis and epithelial histogenesis during tooth development in vivo, but little is known about the inductive potential of dissociated mesenchymal cells used in ex vivo cultures. A 2-step method is described in which, by using different types of reassociations between epithelial and mesenchymal tissues and/or cells from mouse embryos, reassociations were cultured in vitro before in vivo implantation. In vitro, the reassociated tissues developed and resulted in tooth-like structures that exhibited normal epithelial histogenesis and allowed the functional differentiation of odontoblasts and ameloblasts. After implantation, the reassociations formed roots and periodontal ligament, the latter connected to developing bone. The shape of the crown, initially suspected to depend on the integrity of the mesenchyme, could be modulated by adjusting the number of dissociated mesenchymal cells reassociated with the epithelial compartment. Based on these results, we propose a refined strategy for tooth tissue engineering that may help to eventually generate morphologically defined teeth. IS - 8 JF - Tissue engineering SN - 1076-3279 EP - 2075 TI - Tissue engineering of tooth crown, root, and periodontium. VL - 12 SP - 2069 KW - bioengineered_tooth KW - crown KW - periodontium KW - root AU - Hu, Bing AU - Nadiri, Amal AU - Kuchler-Bopp, Sabine AU - Perrin-Schmitt, Fabienne AU - Peters, Heiko AU - Lesot, Hervé PY - 2006/08// UR - http://dx.doi.org/10.1089/ten.2006.12.2069 ER -