Single nucleotide polymorphism array analysis of chromosomal instability patterns discriminates rectal adenomas from carcinomas Export

@article{citeulike:1294607, abstract = {Total mesorectal excision (TME) is the standard treatment for rectal cancer, while transanal endoscopic microsurgery (TEM) is a recently introduced surgical approach for the treatment of rectal adenomas. Incorrect preoperative staging before TEM is a problem. To identify genetic changes that might correlate with tumour stage and could lead to optimized treatment selection we performed a genome-wide chromosomal instability search in a homogeneous, clinical cohort of rectal tumours. 78 rectal tumours during different clinical stages were analysed with 10K single nucleotide polymorphism (SNP) arrays. Logistic regression was performed to build a quantitative model of specific chromosomal aberrations. Overall, most cases (95\%) had one or more chromosomal aberrations. We observed a clear correlation between the total number of aberrations and the different tumour stages. Specifically, the chromosomal events: gain of 8q22-24, 13q and 20q, and loss of 17p and 18q12-22, were far more abundant in carcinoma than in adenoma. In adenoma fractions from cases with a carcinoma (infiltrating at least in the submucosa), twice the amount of such ?malignant aberrations? was observed, compared to pure adenomas. Furthermore, combined aberrations such as gain of 13q and loss of 18q were only found in adenomatous fractions of carcinomas and not in benign lesions. Based on these five genomic events associated with carcinoma, a clear distinction between adenoma and carcinoma tissue could be made. These data should be validated further in order that they may be used in preoperative staging of rectal tumours. Copyright {\copyright} 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley \& Sons, Ltd.}, address = {Department of Pathology, Leiden University Medical Centre, The Netherlands; Department of Surgery, Leiden University Medical Centre, The Netherlands; Department of Molecular Cell Biology, Leiden University Medical Centre, The Netherlands; Department of Medical Statistics, Leiden University Medical Centre, The Netherlands; IJsseland Hospital, The Netherlands; Reinier de Graaf Gasthuis, The Netherlands; Department of Regeneration Medicine for Haematopoiesis, Graduate School of Medicine, University of Tokyo, Japan}, author = {Lips, E. H. and de Graaf, E. J. and Tollenaar, R. A. E. M. and van Eijk, R. and Oosting, J. and Szuhai, K. and Karsten, T. and Nanya, Y. and Ogawa, S. and van de Velde, C. J. and Eilers, P. H. C. and van Wezel, Tom and Morreau, H.}, citeulike-article-id = {1294607}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/path.2180}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17471469}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17471469}, citeulike-linkout-3 = {http://www3.interscience.wiley.com/cgi-bin/abstract/114229576/ABSTRACT}, doi = {10.1002/path.2180}, journal = {The Journal of Pathology}, keywords = {array, cancer, colon, snp}, number = {9999}, pages = {n/a+}, posted-at = {2007-05-14 09:57:16}, priority = {2}, title = {Single nucleotide polymorphism array analysis of chromosomal instability patterns discriminates rectal adenomas from carcinomas}, url = {http://dx.doi.org/10.1002/path.2180}, volume = {9999}, year = {2007} }

TY - JOUR ID - citeulike:1294607 L3 - citeulike-article-id:1294607 N2 - Total mesorectal excision (TME) is the standard treatment for rectal cancer, while transanal endoscopic microsurgery (TEM) is a recently introduced surgical approach for the treatment of rectal adenomas. Incorrect preoperative staging before TEM is a problem. To identify genetic changes that might correlate with tumour stage and could lead to optimized treatment selection we performed a genome-wide chromosomal instability search in a homogeneous, clinical cohort of rectal tumours. 78 rectal tumours during different clinical stages were analysed with 10K single nucleotide polymorphism (SNP) arrays. Logistic regression was performed to build a quantitative model of specific chromosomal aberrations. Overall, most cases (95%) had one or more chromosomal aberrations. We observed a clear correlation between the total number of aberrations and the different tumour stages. Specifically, the chromosomal events: gain of 8q22-24, 13q and 20q, and loss of 17p and 18q12-22, were far more abundant in carcinoma than in adenoma. In adenoma fractions from cases with a carcinoma (infiltrating at least in the submucosa), twice the amount of such ?malignant aberrations? was observed, compared to pure adenomas. Furthermore, combined aberrations such as gain of 13q and loss of 18q were only found in adenomatous fractions of carcinomas and not in benign lesions. Based on these five genomic events associated with carcinoma, a clear distinction between adenoma and carcinoma tissue could be made. These data should be validated further in order that they may be used in preoperative staging of rectal tumours. Copyright © 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. IS - 9999 JF - The Journal of Pathology AD - Department of Pathology, Leiden University Medical Centre, The Netherlands; Department of Surgery, Leiden University Medical Centre, The Netherlands; Department of Molecular Cell Biology, Leiden University Medical Centre, The Netherlands; Department of Medical Statistics, Leiden University Medical Centre, The Netherlands; IJsseland Hospital, The Netherlands; Reinier de Graaf Gasthuis, The Netherlands; Department of Regeneration Medicine for Haematopoiesis, Graduate School of Medicine, University of Tokyo, Japan TI - Single nucleotide polymorphism array analysis of chromosomal instability patterns discriminates rectal adenomas from carcinomas VL - 9999 SP - n/a KW - array KW - cancer KW - colon KW - snp AU - Lips, EH AU - de Graaf, EJ AU - Tollenaar, RAEM AU - van Eijk, R AU - Oosting, J AU - Szuhai, K AU - Karsten, T AU - Nanya, Y AU - Ogawa, S AU - van de Velde, CJ AU - Eilers, PHC AU - van Wezel, Tom AU - Morreau, H PY - 2007/// UR - http://dx.doi.org/10.1002/path.2180 ER -