MUTYH-Null Mice Are Susceptible to Spontaneous and Oxidative Stress Induced Intestinal Tumorigenesis Export

@article{citeulike:1647276, abstract = {MUTYH is a mammalian DNA glycosylase that initiates base excision repair by excising adenine opposite 8-oxoguanine and 2-hydroxyadenine opposite guanine, thereby preventing G:C to T:A transversion caused by oxidative stress. Recently, biallelic germ-line mutations of MUTYH have been found in patients predisposed to a recessive form of hereditary multiple colorectal adenoma and carcinoma with an increased incidence of G:C to T:A somatic mutations in the APC gene. In the present study, a systematic histologic examination revealed that more spontaneous tumors had developed in MUTYH-null mice (72 of 121; 59.5\%) than in the wild type (38 of 109; 34.9\%). The increased incidence of intestinal tumors in MUTYH-null mice (11 tumors in 10 of 121 mice) was statistically significant compared with the wild type (no intestinal tumors in 109 mice). Two adenomas and seven adenocarcinomas were observed in the small intestines, and two adenomas but no carcinomas were found in the colons. In MUTYH-null mice treated with KBrO3, the occurrence of small intestinal tumors dramatically increased. The mean number of polyps induced in the small intestines of these mice was 61.88 (males, 72.75; females, 51.00), whereas it was 0.85 (males, 0.50; females, 1.00) in wild-type mice. The tumors developed predominantly in the duodenum and in the upper region of the (jejunum) small intestines. We conclude that MUTYH suppresses spontaneous tumorigenesis in mammals, thus providing experimental evidence for the association between biallelic germ-line MUTYH mutations and a recessive form of human hereditary colorectal adenoma and carcinoma. [Cancer Res 2007;67(14):6599604] 10.1158/0008-5472.CAN-06-4802}, author = {Sakamoto, Katsumi and Tominaga, Yohei and Yamauchi, Kazumi and Nakatsu, Yoshimichi and Sakumi, Kunihiko and Yoshiyama, Kaoru and Egashira, Akinori and Kura, Shinobu and Yao, Takashi and Tsuneyoshi, Masazumi and Maki, Hisaji and Nakabeppu, Yusaku and Tsuzuki, Teruhisa}, citeulike-article-id = {1647276}, citeulike-linkout-0 = {http://dx.doi.org/10.1158/0008-5472.CAN-06-4802}, citeulike-linkout-1 = {http://cancerres.aacrjournals.org/cgi/content/abstract/67/14/6599}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17638869}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17638869}, day = {15}, doi = {10.1158/0008-5472.CAN-06-4802}, journal = {Cancer Res}, keywords = {muthy}, month = {July}, number = {14}, pages = {6599--6604}, posted-at = {2007-09-12 11:27:44}, priority = {2}, title = {MUTYH-Null Mice Are Susceptible to Spontaneous and Oxidative Stress Induced Intestinal Tumorigenesis}, url = {http://dx.doi.org/10.1158/0008-5472.CAN-06-4802}, volume = {67}, year = {2007} }

TY - JOUR ID - citeulike:1647276 L3 - citeulike-article-id:1647276 N2 - MUTYH is a mammalian DNA glycosylase that initiates base excision repair by excising adenine opposite 8-oxoguanine and 2-hydroxyadenine opposite guanine, thereby preventing G:C to T:A transversion caused by oxidative stress. Recently, biallelic germ-line mutations of MUTYH have been found in patients predisposed to a recessive form of hereditary multiple colorectal adenoma and carcinoma with an increased incidence of G:C to T:A somatic mutations in the APC gene. In the present study, a systematic histologic examination revealed that more spontaneous tumors had developed in MUTYH-null mice (72 of 121; 59.5%) than in the wild type (38 of 109; 34.9%). The increased incidence of intestinal tumors in MUTYH-null mice (11 tumors in 10 of 121 mice) was statistically significant compared with the wild type (no intestinal tumors in 109 mice). Two adenomas and seven adenocarcinomas were observed in the small intestines, and two adenomas but no carcinomas were found in the colons. In MUTYH-null mice treated with KBrO3, the occurrence of small intestinal tumors dramatically increased. The mean number of polyps induced in the small intestines of these mice was 61.88 (males, 72.75; females, 51.00), whereas it was 0.85 (males, 0.50; females, 1.00) in wild-type mice. The tumors developed predominantly in the duodenum and in the upper region of the (jejunum) small intestines. We conclude that MUTYH suppresses spontaneous tumorigenesis in mammals, thus providing experimental evidence for the association between biallelic germ-line MUTYH mutations and a recessive form of human hereditary colorectal adenoma and carcinoma. [Cancer Res 2007;67(14):6599604] 10.1158/0008-5472.CAN-06-4802 IS - 14 JF - Cancer Res EP - 6604 TI - MUTYH-Null Mice Are Susceptible to Spontaneous and Oxidative Stress Induced Intestinal Tumorigenesis VL - 67 SP - 6599 KW - muthy AU - Sakamoto, Katsumi AU - Tominaga, Yohei AU - Yamauchi, Kazumi AU - Nakatsu, Yoshimichi AU - Sakumi, Kunihiko AU - Yoshiyama, Kaoru AU - Egashira, Akinori AU - Kura, Shinobu AU - Yao, Takashi AU - Tsuneyoshi, Masazumi AU - Maki, Hisaji AU - Nakabeppu, Yusaku AU - Tsuzuki, Teruhisa PY - 2007/07/15/ UR - http://dx.doi.org/10.1158/0008-5472.CAN-06-4802 ER -