Oleic acid induces endothelin-1 expression through activation of protein kinase C and NF-kappa B. Export

@article{citeulike:270637, abstract = {This study investigated the effect of oleic acid on the expression levels of endothelin-1 (ET-1) and on the signaling pathways mediating it in human aortic endothelial cells (HAECs). ET-1 mRNA expression was significantly increased by oleic acid in a dose- and time-dependent manner. Elevation of ET-1 expression in response to oleic acid was inhibited by the protein kinase C (PKC) inhibitor, GF109203X, or the NF-kappa B inhibitor, pyrrolidine dithiocarbamate. In addition, both PKC and NF-kappa B activities were significantly increased by oleic acid. Immunoblot analysis revealed that conventional PKCs (PKC-alpha and -beta II isoforms) were significantly increased in the membranous fractions of HAECs treated with oleic acid. PKC inhibitor completely abolished oleic acid-induced NF-kappa B activation, suggesting that PKC activation is upstream of NF-kappa B activation in oleic acid-induced ET-1 expression. These data suggest that elevated plasma oleic acid levels observed in obese, insulin-resistant subjects result in endothelial dysfunction, at least in part, through an increase in ET-1 expression.}, address = {Department of Internal Medicine, University of Ulsan College of Medicine, Poongnap-dong, Songpa-ku, 138-736, Seoul, South Korea}, author = {Park, J. Y. and Kim, Y. M. and Song, H. S. and Park, K. Y. and Kim, Y. M. and Kim, M. S. and Pak, Y. K. and Lee, I. K. and Lee, J. D. and Park, S. J. and Lee, K. U.}, citeulike-article-id = {270637}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12670494}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12670494}, day = {11}, issn = {0006-291X}, journal = {Biochem Biophys Res Commun}, keywords = {endothelin, fattyacids, ffa, nfkb, oleic, proteinkinasec}, month = {April}, number = {3}, pages = {891--895}, posted-at = {2005-08-01 09:33:19}, priority = {2}, title = {Oleic acid induces endothelin-1 expression through activation of protein kinase C and NF-kappa B.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12670494}, volume = {303}, year = {2003} }

TY - JOUR ID - citeulike:270637 L3 - citeulike-article-id:270637 N2 - This study investigated the effect of oleic acid on the expression levels of endothelin-1 (ET-1) and on the signaling pathways mediating it in human aortic endothelial cells (HAECs). ET-1 mRNA expression was significantly increased by oleic acid in a dose- and time-dependent manner. Elevation of ET-1 expression in response to oleic acid was inhibited by the protein kinase C (PKC) inhibitor, GF109203X, or the NF-kappa B inhibitor, pyrrolidine dithiocarbamate. In addition, both PKC and NF-kappa B activities were significantly increased by oleic acid. Immunoblot analysis revealed that conventional PKCs (PKC-alpha and -beta II isoforms) were significantly increased in the membranous fractions of HAECs treated with oleic acid. PKC inhibitor completely abolished oleic acid-induced NF-kappa B activation, suggesting that PKC activation is upstream of NF-kappa B activation in oleic acid-induced ET-1 expression. These data suggest that elevated plasma oleic acid levels observed in obese, insulin-resistant subjects result in endothelial dysfunction, at least in part, through an increase in ET-1 expression. IS - 3 JF - Biochem Biophys Res Commun SN - 0006-291X AD - Department of Internal Medicine, University of Ulsan College of Medicine, Poongnap-dong, Songpa-ku, 138-736, Seoul, South Korea EP - 895 TI - Oleic acid induces endothelin-1 expression through activation of protein kinase C and NF-kappa B. VL - 303 SP - 891 KW - endothelin KW - fattyacids KW - ffa KW - nfkb KW - oleic KW - proteinkinasec AU - Park, JY AU - Kim, YM AU - Song, HS AU - Park, KY AU - Kim, YM AU - Kim, MS AU - Pak, YK AU - Lee, IK AU - Lee, JD AU - Park, SJ AU - Lee, KU PY - 2003/04/11/ UR - http://view.ncbi.nlm.nih.gov/pubmed/12670494 ER -