Biopsy coupled to quantitative immunofluorescence: a new method to study the human vascular endothelium. Export

@article{citeulike:282274, abstract = {Limited availability of endothelial tissue is a major constraint when investigating the cellular mechanisms of endothelial dysfunction in patients with metabolic and cardiovascular diseases. We propose a novel approach that combines collection of 200-1,000 endothelial cells from a superficial forearm vein or the radial artery, with reliable measurements of protein expression by quantitative immunofluorescence analysis. This method was validated against immunoblot analysis in cultured endothelial cells. Levels of vascular endothelial cell activation, oxidative stress, and nitric oxide synthase expression were measured and compared in five patients with severe chronic heart failure and in four healthy age-matched subjects. In summary, vascular endothelial biopsy coupled with measurement of protein expression by quantitative immunofluorescence analysis provides a novel approach to the study of the vascular endothelium in humans.}, address = {Department of Medicine, Division of Cardiology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. lejemtel@aecom.yu.edu}, author = {Colombo, P. C. and Ashton, A. W. and Celaj, S. and Talreja, A. and Banchs, J. E. and Dubois, N. B. and Marinaccio, M. and Malla, S. and Lachmann, J. and Ware, J. A. and Le Jemtel, T. H.}, citeulike-article-id = {282274}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/japplphysiol.00680.2001}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11842075}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11842075}, doi = {10.1152/japplphysiol.00680.2001}, issn = {8750-7587}, journal = {J Appl Physiol}, keywords = {biopsy, endothelialbiopsy, endothelium}, month = {March}, number = {3}, pages = {1331--1338}, posted-at = {2005-08-15 13:47:28}, priority = {2}, title = {Biopsy coupled to quantitative immunofluorescence: a new method to study the human vascular endothelium.}, url = {http://dx.doi.org/10.1152/japplphysiol.00680.2001}, volume = {92}, year = {2002} }

TY - JOUR ID - citeulike:282274 L3 - citeulike-article-id:282274 N2 - Limited availability of endothelial tissue is a major constraint when investigating the cellular mechanisms of endothelial dysfunction in patients with metabolic and cardiovascular diseases. We propose a novel approach that combines collection of 200-1,000 endothelial cells from a superficial forearm vein or the radial artery, with reliable measurements of protein expression by quantitative immunofluorescence analysis. This method was validated against immunoblot analysis in cultured endothelial cells. Levels of vascular endothelial cell activation, oxidative stress, and nitric oxide synthase expression were measured and compared in five patients with severe chronic heart failure and in four healthy age-matched subjects. In summary, vascular endothelial biopsy coupled with measurement of protein expression by quantitative immunofluorescence analysis provides a novel approach to the study of the vascular endothelium in humans. IS - 3 JF - J Appl Physiol SN - 8750-7587 AD - Department of Medicine, Division of Cardiology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. lejemtel@aecom.yu.edu EP - 1338 TI - Biopsy coupled to quantitative immunofluorescence: a new method to study the human vascular endothelium. VL - 92 SP - 1331 KW - biopsy KW - endothelialbiopsy KW - endothelium AU - Colombo, PC AU - Ashton, AW AU - Celaj, S AU - Talreja, A AU - Banchs, JE AU - Dubois, NB AU - Marinaccio, M AU - Malla, S AU - Lachmann, J AU - Ware, JA AU - Le Jemtel, TH PY - 2002/03// UR - http://dx.doi.org/10.1152/japplphysiol.00680.2001 ER -