A universal framework for 13C metabolic flux analysis. Export

@article{citeulike:3335168, abstract = {A general methodology is presented for the modeling, simulation, design, evaluation, and statistical analysis of (13)C-labeling experiments for metabolic flux analysis. The universal software framework 13C-FLUX was implemented to support all steps of this process. Guided by the example of anaplerotic flux determination in Corynebacterium glutamicum, the technical details of the model setup, experimental design, and data evaluation are discussed. It is shown how the network structure, the input substrate composition, the assumptions about fluxes, and the measurement configuration are specified within 13C-FLUX. Based on the network model, different experimental designs are computed depending on the goal of the investigations. Finally, a specific experiment is evaluated and the various statistical methods used to analyze the results are briefly explained. The appendix gives some details about the software implementation and availability.}, address = {Department of Simulation, IMR, University of Siegen, Paul-Bonatz-Strasse 9-11, D-57068 Siegen, Germany. wiechert@simtec.mb.uni-siegen.de}, author = {Wiechert, W. and M\"{o}llney, M. and Petersen, S. and de Graaf, A. A.}, citeulike-article-id = {3335168}, citeulike-linkout-0 = {http://dx.doi.org/10.1006/mben.2001.0188}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11461148}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11461148}, doi = {10.1006/mben.2001.0188}, issn = {1096-7176}, journal = {Metabolic engineering}, keywords = {13c, analysis, flux, metabolic}, month = {July}, number = {3}, pages = {265--283}, posted-at = {2008-09-25 10:41:29}, priority = {2}, title = {A universal framework for 13C metabolic flux analysis.}, url = {http://dx.doi.org/10.1006/mben.2001.0188}, volume = {3}, year = {2001} }

TY - JOUR ID - citeulike:3335168 L3 - citeulike-article-id:3335168 N2 - A general methodology is presented for the modeling, simulation, design, evaluation, and statistical analysis of (13)C-labeling experiments for metabolic flux analysis. The universal software framework 13C-FLUX was implemented to support all steps of this process. Guided by the example of anaplerotic flux determination in Corynebacterium glutamicum, the technical details of the model setup, experimental design, and data evaluation are discussed. It is shown how the network structure, the input substrate composition, the assumptions about fluxes, and the measurement configuration are specified within 13C-FLUX. Based on the network model, different experimental designs are computed depending on the goal of the investigations. Finally, a specific experiment is evaluated and the various statistical methods used to analyze the results are briefly explained. The appendix gives some details about the software implementation and availability. IS - 3 JF - Metabolic engineering SN - 1096-7176 AD - Department of Simulation, IMR, University of Siegen, Paul-Bonatz-Strasse 9-11, D-57068 Siegen, Germany. wiechert@simtec.mb.uni-siegen.de EP - 283 TI - A universal framework for 13C metabolic flux analysis. VL - 3 SP - 265 KW - 13c KW - analysis KW - flux KW - metabolic AU - Wiechert, W AU - Möllney, M AU - Petersen, S AU - de Graaf, AA PY - 2001/07// UR - http://dx.doi.org/10.1006/mben.2001.0188 ER -