Genotyping by Oligonucleotide Ligation Assay (OLA) Export

@article{citeulike:1620757, abstract = {This protocol describes the oligonucleotide ligation assay (OLA), which uses a set of three oligonucleotides, in combination with a thermostable Taq DNA ligase enzyme, to discriminate single-nucleotide polymorphism (SNP) alleles. Sixteen-plex OLA genotyping reactions are carried out, and allele-specific OLA products are detected on membrane arrays using radiolabeled probes. 10.1101/pdb.prot4843}, author = {Macdonald, Stuart J.}, citeulike-article-id = {1620757}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/pdb.prot4843}, citeulike-linkout-1 = {http://www.cshprotocols.org/cgi/content/abstract/2007/18/pdb.prot4843}, day = {1}, doi = {10.1101/pdb.prot4843}, journal = {Cold Spring Harbor Protocols}, keywords = {bioinformatics, biology, gene, genetic, genomics, method, methods, molecular, protocol, sequence, snp, variation}, month = {September}, number = {18}, pages = {pdb.prot4843+}, posted-at = {2007-09-04 21:05:40}, priority = {0}, title = {Genotyping by Oligonucleotide Ligation Assay (OLA)}, url = {http://dx.doi.org/10.1101/pdb.prot4843}, volume = {2007}, year = {2007} }

TY - JOUR ID - citeulike:1620757 L3 - citeulike-article-id:1620757 N2 - This protocol describes the oligonucleotide ligation assay (OLA), which uses a set of three oligonucleotides, in combination with a thermostable Taq DNA ligase enzyme, to discriminate single-nucleotide polymorphism (SNP) alleles. Sixteen-plex OLA genotyping reactions are carried out, and allele-specific OLA products are detected on membrane arrays using radiolabeled probes. 10.1101/pdb.prot4843 IS - 18 JF - Cold Spring Harbor Protocols TI - Genotyping by Oligonucleotide Ligation Assay (OLA) VL - 2007 SP - pdb.prot4843 KW - bioinformatics KW - biology KW - gene KW - genetic KW - genomics KW - method KW - methods KW - molecular KW - protocol KW - sequence KW - snp KW - variation AU - Macdonald, Stuart PY - 2007/09/01/ UR - http://dx.doi.org/10.1101/pdb.prot4843 ER -