Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi) Export

@article{citeulike:2319868, abstract = {RNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a technique by which Drosophila embryos can be injected with dsRNA in order to disrupt targeted gene function. The approach is straightforward, utilizing improved methods for injecting the dsRNA directly through the chorion of the embryo. This strategy minimizes problems normally associated with desiccation of the dechorionated embryo and facilitates post-injection analysis of gene expression. 10.1101/pdb.prot4918}, author = {Misquitta, Leonie and Wei, Qin and Paterson, Bruce M.}, citeulike-article-id = {2319868}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/pdb.prot4918}, citeulike-linkout-1 = {http://www.cshprotocols.org/cgi/content/abstract/2008/3/pdb.prot4918}, day = {1}, doi = {10.1101/pdb.prot4918}, journal = {Cold Spring Harbor Protocols}, keywords = {biology, development, dna, drosophila, genetic, laboratory, method, methods, molecular, protocol, protocols, rna, rnai, silencing, sirna}, month = {February}, number = {3}, pages = {pdb.prot4918+}, posted-at = {2008-02-01 15:53:30}, priority = {0}, title = {Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)}, url = {http://dx.doi.org/10.1101/pdb.prot4918}, volume = {2008}, year = {2008} }

TY - JOUR ID - citeulike:2319868 L3 - citeulike-article-id:2319868 N2 - RNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a technique by which Drosophila embryos can be injected with dsRNA in order to disrupt targeted gene function. The approach is straightforward, utilizing improved methods for injecting the dsRNA directly through the chorion of the embryo. This strategy minimizes problems normally associated with desiccation of the dechorionated embryo and facilitates post-injection analysis of gene expression. 10.1101/pdb.prot4918 IS - 3 JF - Cold Spring Harbor Protocols TI - Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi) VL - 2008 SP - pdb.prot4918 KW - biology KW - development KW - dna KW - drosophila KW - genetic KW - laboratory KW - method KW - methods KW - molecular KW - protocol KW - protocols KW - rna KW - rnai KW - silencing KW - sirna AU - Misquitta, Leonie AU - Wei, Qin AU - Paterson, Bruce PY - 2008/02/01/ UR - http://dx.doi.org/10.1101/pdb.prot4918 ER -