Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation Export

@article{citeulike:2522270, abstract = {This protocol describes the use of methylated CpG island amplification (MCA) in combination with a microarray platform to analyze genome-wide DNA methylation in a high-throughput fashion. In this approach, termed MCAM, methylated CpG islands are selectively targeted using oligonucleotide adaptors after two rounds of digestion with a combination of methylation-sensitive and methylation-insensitive nucleases. They are then amplified using PCR. The resulting amplicons, representing the methylated fraction of the genome, are labeled with fluorochromes. Subsequently, a comparative hybridization of reference and test samples (typically normal and tumor DNA specimens) is done on a microarray platform. 10.1101/pdb.prot4974}, author = {Estecio, Marcos R. and Yan, Pearlly S. and Huang, Tim H. and Issa, Jean-Pierre J.}, citeulike-article-id = {2522270}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/pdb.prot4974}, citeulike-linkout-1 = {http://www.cshprotocols.org/cgi/content/abstract/2008/4/pdb.prot4974}, day = {1}, doi = {10.1101/pdb.prot4974}, journal = {Cold Spring Harbor Protocols}, keywords = {bioinformatics, biology, chromosome, dna, epigenetics, genetic, genomics, histone, laboratory, method, methods, methylation, modification, molecular, protocol, protocols, regulation, transcription}, month = {March}, number = {4}, pages = {pdb.prot4974+}, posted-at = {2008-03-12 19:16:07}, priority = {0}, title = {Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation}, url = {http://dx.doi.org/10.1101/pdb.prot4974}, volume = {2008}, year = {2008} }

TY - JOUR ID - citeulike:2522270 L3 - citeulike-article-id:2522270 N2 - This protocol describes the use of methylated CpG island amplification (MCA) in combination with a microarray platform to analyze genome-wide DNA methylation in a high-throughput fashion. In this approach, termed MCAM, methylated CpG islands are selectively targeted using oligonucleotide adaptors after two rounds of digestion with a combination of methylation-sensitive and methylation-insensitive nucleases. They are then amplified using PCR. The resulting amplicons, representing the methylated fraction of the genome, are labeled with fluorochromes. Subsequently, a comparative hybridization of reference and test samples (typically normal and tumor DNA specimens) is done on a microarray platform. 10.1101/pdb.prot4974 IS - 4 JF - Cold Spring Harbor Protocols TI - Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation VL - 2008 SP - pdb.prot4974 KW - bioinformatics KW - biology KW - chromosome KW - dna KW - epigenetics KW - genetic KW - genomics KW - histone KW - laboratory KW - method KW - methods KW - methylation KW - modification KW - molecular KW - protocol KW - protocols KW - regulation KW - transcription AU - Estecio, Marcos AU - Yan, Pearlly AU - Huang, Tim AU - Issa, Jean-Pierre PY - 2008/03/01/ UR - http://dx.doi.org/10.1101/pdb.prot4974 ER -