qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Export

@article{citeulike:1099286, abstract = {Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.}, author = {Hellemans, Jan and Mortier, Geert and De Paepe, Anne and Speleman, Frank and Vandesompele, Jo}, citeulike-article-id = {1099286}, citeulike-linkout-0 = {http://dx.doi.org/10.1186/gb-2007-8-2-r19}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17291332}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17291332}, day = {09}, doi = {10.1186/gb-2007-8-2-r19}, issn = {1465-6914}, journal = {Genome biology}, keywords = {expression, mining, silico, stat}, month = {February}, number = {2}, pages = {R19+}, posted-at = {2009-10-26 16:43:51}, priority = {2}, title = {qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data.}, url = {http://dx.doi.org/10.1186/gb-2007-8-2-r19}, volume = {8}, year = {2007} }

TY - JOUR ID - citeulike:1099286 L3 - citeulike-article-id:1099286 N2 - Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data. IS - 2 JF - Genome biology SN - 1465-6914 TI - qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. VL - 8 SP - R19 KW - expression KW - mining KW - silico KW - stat AU - Hellemans, Jan AU - Mortier, Geert AU - De Paepe, Anne AU - Speleman, Frank AU - Vandesompele, Jo PY - 2007/02/09/ UR - http://dx.doi.org/10.1186/gb-2007-8-2-r19 ER -