The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Export

@article{citeulike:4139423, abstract = {BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments. CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement. SUMMARY: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.}, author = {Bustin, Stephen A. and Benes, Vladimir and Garson, Jeremy A. and Hellemans, Jan and Huggett, Jim and Kubista, Mikael and Mueller, Reinhold and Nolan, Tania and Pfaffl, Michael W. and Shipley, Gregory L. and Vandesompele, Jo and Wittwer, Carl T.}, citeulike-article-id = {4139423}, citeulike-linkout-0 = {http://dx.doi.org/10.1373/clinchem.2008.112797}, citeulike-linkout-1 = {http://www.clinchem.org/content/55/4/611.abstract}, citeulike-linkout-2 = {http://www.clinchem.org/content/55/4/611.full.pdf}, citeulike-linkout-3 = {http://www.clinchem.org/cgi/content/abstract/55/4/611}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/19246619}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=19246619}, day = {1}, doi = {10.1373/clinchem.2008.112797}, issn = {1530-8561}, journal = {Clinical chemistry}, keywords = {expression, highly, metaadpd, silico}, month = {April}, number = {4}, pages = {611--622}, posted-at = {2009-10-26 16:43:23}, priority = {2}, title = {The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.}, url = {http://dx.doi.org/10.1373/clinchem.2008.112797}, volume = {55}, year = {2009} }

TY - JOUR ID - citeulike:4139423 L3 - citeulike-article-id:4139423 N2 - BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments. CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement. SUMMARY: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results. IS - 4 JF - Clinical chemistry SN - 1530-8561 EP - 622 TI - The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. VL - 55 SP - 611 KW - expression KW - highly KW - metaadpd KW - silico AU - Bustin, Stephen AU - Benes, Vladimir AU - Garson, Jeremy AU - Hellemans, Jan AU - Huggett, Jim AU - Kubista, Mikael AU - Mueller, Reinhold AU - Nolan, Tania AU - Pfaffl, Michael AU - Shipley, Gregory AU - Vandesompele, Jo AU - Wittwer, Carl PY - 2009/04/01/ UR - http://dx.doi.org/10.1373/clinchem.2008.112797 ER -