A functional polymorphism in Pre-miR-146a gene is associated with prostate cancer risk and mature miR-146a expression in vivo. Export

@article{citeulike:6102258, abstract = {BACKGROUND: A G > C polymorphism (rs2910164) which is located in the sequence of miR-146a precursor, results in a change from a G:U pair to a C:U mismatch in its stem region. To explore whether rs2910164 plays any role in prostate cancer (CaP), we analyzed the association between miR-146a polymorphism and risk of CaP and the expression of miR-146a with different genotypes in CaP tissues in southern Chinese Han population. MATERIALS AND METHODS: Two hundred fifty-one CaP and 280 control subjects were included in the cancer association study, and 15 CaP tissue samples were used to test the expression of the miRNA precursors by real-time quantitative reverse transcription PCR. RESULTS: We found that subjects carrying CC homozygotes had a 0.65-fold reduced risk (95\% CI = 0.43-0.99) than those carrying GG/GC genotypes (P = 0.03), and the C allele displayed a lower prevalence of CaP compared with the G allele (OR = 0.73, 95\% CI = 0.57-0.94, P = 0.01). Moreover, hsa-miR-146a quantification showed that homozygous carriers of the C-variant had significantly decreased miRNA levels compared to the carriers of the GG/GC genotype. CONCLUSIONS: The natural genetic variation in pre-miR-146a affects the amount of mature miR-146a, contributes to the genetic predisposition to CaP. Prostate (c) 2009 Wiley-Liss, Inc.}, author = {Xu, Bin and Feng, Ning-Han H. and Li, Peng-Chao C. and Tao, Jun and Wu, Deyao and Zhang, Zheng-Dong D. and Tong, Na and Wang, Jin-Feng F. and Song, Ning-Hong H. and Zhang, Wei and Hua, Li-Xin X. and Wu, Hong-Fei F.}, citeulike-article-id = {6102258}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/pros.21080}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19902466}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19902466}, day = {9}, doi = {10.1002/pros.21080}, issn = {1097-0045}, journal = {The Prostate}, keywords = {assoc, humgen, mirna}, month = {November}, posted-at = {2009-11-12 15:49:17}, priority = {2}, title = {A functional polymorphism in Pre-miR-146a gene is associated with prostate cancer risk and mature miR-146a expression in vivo.}, url = {http://dx.doi.org/10.1002/pros.21080}, year = {2009} }

TY - JOUR ID - citeulike:6102258 L3 - citeulike-article-id:6102258 N2 - BACKGROUND: A G > C polymorphism (rs2910164) which is located in the sequence of miR-146a precursor, results in a change from a G:U pair to a C:U mismatch in its stem region. To explore whether rs2910164 plays any role in prostate cancer (CaP), we analyzed the association between miR-146a polymorphism and risk of CaP and the expression of miR-146a with different genotypes in CaP tissues in southern Chinese Han population. MATERIALS AND METHODS: Two hundred fifty-one CaP and 280 control subjects were included in the cancer association study, and 15 CaP tissue samples were used to test the expression of the miRNA precursors by real-time quantitative reverse transcription PCR. RESULTS: We found that subjects carrying CC homozygotes had a 0.65-fold reduced risk (95% CI = 0.43-0.99) than those carrying GG/GC genotypes (P = 0.03), and the C allele displayed a lower prevalence of CaP compared with the G allele (OR = 0.73, 95% CI = 0.57-0.94, P = 0.01). Moreover, hsa-miR-146a quantification showed that homozygous carriers of the C-variant had significantly decreased miRNA levels compared to the carriers of the GG/GC genotype. CONCLUSIONS: The natural genetic variation in pre-miR-146a affects the amount of mature miR-146a, contributes to the genetic predisposition to CaP. Prostate (c) 2009 Wiley-Liss, Inc. JF - The Prostate SN - 1097-0045 TI - A functional polymorphism in Pre-miR-146a gene is associated with prostate cancer risk and mature miR-146a expression in vivo. KW - assoc KW - humgen KW - mirna AU - Xu, Bin AU - Feng, Ning-Han AU - Li, Peng-Chao AU - Tao, Jun AU - Wu, Deyao AU - Zhang, Zheng-Dong AU - Tong, Na AU - Wang, Jin-Feng AU - Song, Ning-Hong AU - Zhang, Wei AU - Hua, Li-Xin AU - Wu, Hong-Fei PY - 2009/11/09/ UR - http://dx.doi.org/10.1002/pros.21080 ER -