Alterations in mesenteric lymph node T cell phenotype and cytokine secretion are associated with changes in thymocyte phenotype after LP-BM5 retrovirus infection. Export

@article{citeulike:1341844, abstract = {In this study, mouse MLN cells and thymocytes from advanced stages of LP-BM5 retrovirus infection were studied. A decrease in the percentage of IL-7(+) cells and an increase in the percentage of IL-16(+) cells in the MLN indicated that secretion of these cytokines was also altered after LP-BM5 infection. The percentage of MLN T cells expressing IL-7 receptors was significantly reduced, while the percentage of MLN T cells expressing TNFR-p75 and of B cells expressing TNFR-p55 increased. Simultaneous analysis of surface markers and cytokine secretion was done in an attempt to understand whether the deregulation of IFN-gamma secretion could be ascribed to a defined cell phenotype, concluding that all T cell subsets studied increased IFN-gamma secretion after retrovirus infection. Finally, thymocyte phenotype was further analyzed trying to correlate changes in thymocyte phenotype with MLN cell phenotype. The results indicated that the increase in single positive either CD4(+)CD8(-) or CD4(-)CD8(+) cells was due to accumulation of both immature (CD3(-)) and mature (CD3(+)) single positive thymocytes. Moreover, single positive mature thymocytes presented a phenotype similar to the phenotype previously seen on MLN T cells. In summary, we can conclude that LP-BM5 uses the immune system to reach the thymus where it interferes with the generation of functionally mature T cells, favoring the development of T cells with an abnormal phenotype. These new T cells are activated to secrete several cytokines that in turn will favor retrovirus replication and inhibit any attempt of the immune system to control infection.}, address = {Health Promotion Sciences, Enid and Mel Zuckerman College of Public Health, The University of Arizona, Tucson, AZ 85724, USA.}, author = {Lopez, M. C. and Watson, R. R.}, citeulike-article-id = {1341844}, citeulike-linkout-0 = {http://dx.doi.org/10.1080/17402520500303339}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16584110}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16584110}, comment = {Mesenteric LN are located in the mesentary of the small intestine and pancreas all retroviruses reach the thymus and interfere with t cell differentiation * LP-BM5 is a murine leukemia retrovirus, it favors proliferation in cd4 cells aso it renders the cd4 cells anergic * after retroviral infection, neither t nor b cells from the MLN could migrate to the gut lamina propia * il-7: crucial cytokine for b cell and t cell development * retroviral infection decreases il7+ cells in MLN, same with IL7R cells * retroviral infection indreases IL16 production- a chemotractant for T cells * IFN- gamma increases during LP-BM5 infection and this increase may not be t he result of a protective immune response, but it may be induced by the retrovirus since animals infected with retrovirus and treated with anti ifn-gamma antibodies do not show severe pathology * LP-bm5 uses the immune system to reach the thymus and there it impairs t cell development, and these t cell release a unique cytokine profile that favors retroviral survival and disallows immune control ---=note-separator=--- Upon infection- MLN increase in size and cell numbers within MLN go up!}, doi = {10.1080/17402520500303339}, issn = {1740-2522}, journal = {Clin Dev Immunol}, keywords = {alteration, cell, cytokine, of, profile, retroviral, t}, month = {December}, number = {4}, pages = {249--257}, posted-at = {2007-05-30 00:03:13}, priority = {0}, title = {Alterations in mesenteric lymph node T cell phenotype and cytokine secretion are associated with changes in thymocyte phenotype after LP-BM5 retrovirus infection.}, url = {http://dx.doi.org/10.1080/17402520500303339}, volume = {12}, year = {2005} }

TY - JOUR ID - citeulike:1341844 L3 - citeulike-article-id:1341844 N2 - In this study, mouse MLN cells and thymocytes from advanced stages of LP-BM5 retrovirus infection were studied. A decrease in the percentage of IL-7(+) cells and an increase in the percentage of IL-16(+) cells in the MLN indicated that secretion of these cytokines was also altered after LP-BM5 infection. The percentage of MLN T cells expressing IL-7 receptors was significantly reduced, while the percentage of MLN T cells expressing TNFR-p75 and of B cells expressing TNFR-p55 increased. Simultaneous analysis of surface markers and cytokine secretion was done in an attempt to understand whether the deregulation of IFN-gamma secretion could be ascribed to a defined cell phenotype, concluding that all T cell subsets studied increased IFN-gamma secretion after retrovirus infection. Finally, thymocyte phenotype was further analyzed trying to correlate changes in thymocyte phenotype with MLN cell phenotype. The results indicated that the increase in single positive either CD4(+)CD8(-) or CD4(-)CD8(+) cells was due to accumulation of both immature (CD3(-)) and mature (CD3(+)) single positive thymocytes. Moreover, single positive mature thymocytes presented a phenotype similar to the phenotype previously seen on MLN T cells. In summary, we can conclude that LP-BM5 uses the immune system to reach the thymus where it interferes with the generation of functionally mature T cells, favoring the development of T cells with an abnormal phenotype. These new T cells are activated to secrete several cytokines that in turn will favor retrovirus replication and inhibit any attempt of the immune system to control infection. IS - 4 JF - Clin Dev Immunol SN - 1740-2522 AD - Health Promotion Sciences, Enid and Mel Zuckerman College of Public Health, The University of Arizona, Tucson, AZ 85724, USA. EP - 257 TI - Alterations in mesenteric lymph node T cell phenotype and cytokine secretion are associated with changes in thymocyte phenotype after LP-BM5 retrovirus infection. VL - 12 SP - 249 KW - alteration KW - cell KW - cytokine KW - of KW - profile KW - retroviral KW - t N1 - Mesenteric LN are located in the mesentary of the small intestine and pancreas all retroviruses reach the thymus and interfere with t cell differentiation * LP-BM5 is a murine leukemia retrovirus, it favors proliferation in cd4 cells aso it renders the cd4 cells anergic * after retroviral infection, neither t nor b cells from the MLN could migrate to the gut lamina propia * il-7: crucial cytokine for b cell and t cell development * retroviral infection decreases il7+ cells in MLN, same with IL7R cells * retroviral infection indreases IL16 production- a chemotractant for T cells * IFN- gamma increases during LP-BM5 infection and this increase may not be t he result of a protective immune response, but it may be induced by the retrovirus since animals infected with retrovirus and treated with anti ifn-gamma antibodies do not show severe pathology * LP-bm5 uses the immune system to reach the thymus and there it impairs t cell development, and these t cell release a unique cytokine profile that favors retroviral survival and disallows immune control N1 - Upon infection- MLN increase in size and cell numbers within MLN go up! AU - Lopez, MC AU - Watson, RR PY - 2005/12// UR - http://dx.doi.org/10.1080/17402520500303339 ER -