Global Changes in Kaposi's Sarcoma-Associated Virus Gene Expression Patterns following Expression of a Tetracycline-Inducible Rta Transactivator Export

@article{citeulike:1915684, abstract = {An important step in the herpesvirus life cycle is the switch from latency to lytic reactivation. In order to study the life cycle of Kaposi's sarcoma-associated herpesvirus (KSHV), we developed a gene expression system in KSHV-infected primary effusion lymphoma cells. This system uses Flp-mediated efficient recombination and tetracycline-inducible expression. The Rta transcriptional activator, which acts as a molecular switch for lytic reactivation of KSHV, was efficiently integrated downstream of the Flp recombination target site, and its expression was tightly controlled by tetracycline. Like stimulation with tetradecanoyl phorbol acetate (TPA), the ectopic expression of Rta efficiently induced a complete cycle of viral replication, including a well-ordered program of KSHV gene expression and production of infectious viral progeny. A striking feature of Rta-mediated lytic gene expression was that Rta induced KSHV gene expression in a more powerful and efficient manner than TPA stimulation, indicating that Rta plays a central, leading role in KSHV lytic gene expression. Thus, our streamlined gene expression system provides a novel means not only to study the effects of viral gene products on overall KSHV gene expression and replication, but also to understand the natural viral reactivation process. 10.1128/JVI.77.7.4205-4220.2003}, author = {Nakamura, Hiroyuki and Lu, Michael and Gwack, Yousang and Souvlis, John and Zeichner, Steven L. and Jung, Jae U.}, citeulike-article-id = {1915684}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JVI.77.7.4205-4220.2003}, citeulike-linkout-1 = {http://jvi.asm.org/cgi/content/abstract/77/7/4205}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12634378}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12634378}, day = {1}, doi = {10.1128/JVI.77.7.4205-4220.2003}, journal = {J. Virol.}, keywords = {bcbl-1rta}, month = {April}, number = {7}, pages = {4205--4220}, posted-at = {2007-11-14 19:54:05}, priority = {2}, title = {Global Changes in Kaposi's Sarcoma-Associated Virus Gene Expression Patterns following Expression of a Tetracycline-Inducible Rta Transactivator}, url = {http://dx.doi.org/10.1128/JVI.77.7.4205-4220.2003}, volume = {77}, year = {2003} }

TY - JOUR ID - citeulike:1915684 L3 - citeulike-article-id:1915684 N2 - An important step in the herpesvirus life cycle is the switch from latency to lytic reactivation. In order to study the life cycle of Kaposi's sarcoma-associated herpesvirus (KSHV), we developed a gene expression system in KSHV-infected primary effusion lymphoma cells. This system uses Flp-mediated efficient recombination and tetracycline-inducible expression. The Rta transcriptional activator, which acts as a molecular switch for lytic reactivation of KSHV, was efficiently integrated downstream of the Flp recombination target site, and its expression was tightly controlled by tetracycline. Like stimulation with tetradecanoyl phorbol acetate (TPA), the ectopic expression of Rta efficiently induced a complete cycle of viral replication, including a well-ordered program of KSHV gene expression and production of infectious viral progeny. A striking feature of Rta-mediated lytic gene expression was that Rta induced KSHV gene expression in a more powerful and efficient manner than TPA stimulation, indicating that Rta plays a central, leading role in KSHV lytic gene expression. Thus, our streamlined gene expression system provides a novel means not only to study the effects of viral gene products on overall KSHV gene expression and replication, but also to understand the natural viral reactivation process. 10.1128/JVI.77.7.4205-4220.2003 IS - 7 JF - J. Virol. EP - 4220 TI - Global Changes in Kaposi's Sarcoma-Associated Virus Gene Expression Patterns following Expression of a Tetracycline-Inducible Rta Transactivator VL - 77 SP - 4205 KW - bcbl-1rta AU - Nakamura, Hiroyuki AU - Lu, Michael AU - Gwack, Yousang AU - Souvlis, John AU - Zeichner, Steven AU - Jung, Jae PY - 2003/04/01/ UR - http://dx.doi.org/10.1128/JVI.77.7.4205-4220.2003 ER -