Functional characterization of Kaposi's sarcoma-associated herpesvirus ORF45 by bacterial artificial chromosome-based mutagenesis. Export

@article{citeulike:2330885, abstract = {Open reading frame 45 (ORF45) of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes an immediate-early protein. This protein is also present in virions as a tegument protein. ORF45 protein interacts with interferon regulatory factor 7 (IRF-7) and inhibits virus-induced type I interferon production by blocking activation of IRF-7. To define further the function of ORF45 and the mechanism underlying its action, we constructed an ORF45-null recombinant virus genome (BAC-stop45) by using a bacterial artificial chromosome (BAC) system. Stable 293T cells carrying the BAC36 (wild type) and BAC-stop45 genomes were generated. When monolayers of 293T BAC36 and 293T BAC-stop45 cells were induced with 12-O-tetradecanoylphorbol-13-acetate and sodium butyrate, no significant difference was found between them in overall viral gene expression and lytic DNA replication, but induced 293T BAC-stop45 cells released 10-fold fewer virions to the medium than did 293T BAC36 cells. When ORF45-null virus was used to infect cells, lower infectivity was observed than for wild-type BAC36. These results suggest that KSHV ORF45 plays roles in both early and late stages of viral infection, probably in viral ingress and egress.}, address = {Department of Microbiology, School of Dental Medicine, University of Pennsylvania, 240 S. 40th Street, Philadelphia, PA 19104, USA.}, author = {Zhu, F. X. and Li, X. and Zhou, F. and Gao, S. J. and Yuan, Y.}, citeulike-article-id = {2330885}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JVI.01275-06}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17035322}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17035322}, doi = {10.1128/JVI.01275-06}, issn = {0022-538X}, journal = {J Virol}, keywords = {bac36, orf45}, month = {December}, number = {24}, pages = {12187--12196}, posted-at = {2008-02-04 21:00:02}, priority = {2}, title = {Functional characterization of Kaposi's sarcoma-associated herpesvirus ORF45 by bacterial artificial chromosome-based mutagenesis.}, url = {http://dx.doi.org/10.1128/JVI.01275-06}, volume = {80}, year = {2006} }

TY - JOUR ID - citeulike:2330885 L3 - citeulike-article-id:2330885 N2 - Open reading frame 45 (ORF45) of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes an immediate-early protein. This protein is also present in virions as a tegument protein. ORF45 protein interacts with interferon regulatory factor 7 (IRF-7) and inhibits virus-induced type I interferon production by blocking activation of IRF-7. To define further the function of ORF45 and the mechanism underlying its action, we constructed an ORF45-null recombinant virus genome (BAC-stop45) by using a bacterial artificial chromosome (BAC) system. Stable 293T cells carrying the BAC36 (wild type) and BAC-stop45 genomes were generated. When monolayers of 293T BAC36 and 293T BAC-stop45 cells were induced with 12-O-tetradecanoylphorbol-13-acetate and sodium butyrate, no significant difference was found between them in overall viral gene expression and lytic DNA replication, but induced 293T BAC-stop45 cells released 10-fold fewer virions to the medium than did 293T BAC36 cells. When ORF45-null virus was used to infect cells, lower infectivity was observed than for wild-type BAC36. These results suggest that KSHV ORF45 plays roles in both early and late stages of viral infection, probably in viral ingress and egress. IS - 24 JF - J Virol SN - 0022-538X AD - Department of Microbiology, School of Dental Medicine, University of Pennsylvania, 240 S. 40th Street, Philadelphia, PA 19104, USA. EP - 12196 TI - Functional characterization of Kaposi's sarcoma-associated herpesvirus ORF45 by bacterial artificial chromosome-based mutagenesis. VL - 80 SP - 12187 KW - bac36 KW - orf45 AU - Zhu, FX AU - Li, X AU - Zhou, F AU - Gao, SJ AU - Yuan, Y PY - 2006/12// UR - http://dx.doi.org/10.1128/JVI.01275-06 ER -