CD4 T cell control of acute and latent murine gammaherpesvirus infection requires IFN[gamma] Export

@article{citeulike:2710082, abstract = {Murine gammaherpesvirus 68 ([gamma]HV68, MHV-68)-specific CD4 T cells control [gamma]HV68 infection by reducing the frequency of latently infected cells and by inhibiting viral replication. We have previously demonstrated that CD4 T cells do not require CD8 T or B cells to control [gamma]HV68 replication, demonstrating a helper-independent activity of CD4 T cells during [gamma]HV68 infection. The effector mechanism(s) required for this helper-independent function of CD4 T cells and for the inhibition of the establishment of latency by CD4 T cells are not known. Since IFN[gamma] has been previously shown to be important for control of acute, latent, and persistent [gamma]HV68 infection, we tested the hypothesis that CD4 T cells require IFN[gamma] to limit [gamma]HV68 latency and replication. We utilized a previously described system in which T cell receptor (TCR) transgenic T cells (DO.11.10) and a recombinant virus ([gamma]HV68.OVA) allow for evaluation of high numbers of virus-specific CD4 T cells during both acute and latent infection. We show here that virus-specific CD4 T cells require IFN[gamma] for their anti-viral function in both acute and latent [gamma]HV68 infection. We additionally show that an in vitro derived T helper type 1 (TH1) CD4 T cell clone, which produces IFN[gamma], inhibits [gamma]HV68 replication after adoptive transfer into RAG mice. Together, data presented here demonstrate that both CD4 T cell-mediated helper-independent control of [gamma]HV68 replication and inhibition of the establishment of [gamma]HV68 latency require IFN[gamma].}, author = {Sparks-Thissen, Rebecca L. and Braaten, Douglas C. and Hildner, Kai and Murphy, Theresa L. and Murphy, Kenneth M. and Virgin}, citeulike-article-id = {2710082}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.virol.2005.05.011}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WXR-4GDBT55-2/1/b5c24712f0bb696f30257b99387883e1}, day = {1}, doi = {10.1016/j.virol.2005.05.011}, journal = {Virology}, keywords = {cd4, cd8, mhv68}, month = {August}, number = {2}, pages = {201--208}, posted-at = {2008-04-23 21:08:03}, priority = {4}, title = {CD4 T cell control of acute and latent murine gammaherpesvirus infection requires IFN[gamma]}, url = {http://dx.doi.org/10.1016/j.virol.2005.05.011}, volume = {338}, year = {2005} }

TY - JOUR ID - citeulike:2710082 L3 - citeulike-article-id:2710082 N2 - Murine gammaherpesvirus 68 ([gamma]HV68, MHV-68)-specific CD4 T cells control [gamma]HV68 infection by reducing the frequency of latently infected cells and by inhibiting viral replication. We have previously demonstrated that CD4 T cells do not require CD8 T or B cells to control [gamma]HV68 replication, demonstrating a helper-independent activity of CD4 T cells during [gamma]HV68 infection. The effector mechanism(s) required for this helper-independent function of CD4 T cells and for the inhibition of the establishment of latency by CD4 T cells are not known. Since IFN[gamma] has been previously shown to be important for control of acute, latent, and persistent [gamma]HV68 infection, we tested the hypothesis that CD4 T cells require IFN[gamma] to limit [gamma]HV68 latency and replication. We utilized a previously described system in which T cell receptor (TCR) transgenic T cells (DO.11.10) and a recombinant virus ([gamma]HV68.OVA) allow for evaluation of high numbers of virus-specific CD4 T cells during both acute and latent infection. We show here that virus-specific CD4 T cells require IFN[gamma] for their anti-viral function in both acute and latent [gamma]HV68 infection. We additionally show that an in vitro derived T helper type 1 (TH1) CD4 T cell clone, which produces IFN[gamma], inhibits [gamma]HV68 replication after adoptive transfer into RAG mice. Together, data presented here demonstrate that both CD4 T cell-mediated helper-independent control of [gamma]HV68 replication and inhibition of the establishment of [gamma]HV68 latency require IFN[gamma]. IS - 2 JF - Virology EP - 208 TI - CD4 T cell control of acute and latent murine gammaherpesvirus infection requires IFN[gamma] VL - 338 SP - 201 KW - cd4 KW - cd8 KW - mhv68 AU - Sparks-Thissen, Rebecca AU - Braaten, Douglas AU - Hildner, Kai AU - Murphy, Theresa AU - Murphy, Kenneth AU - Virgin PY - 2005/08/01/ UR - http://dx.doi.org/10.1016/j.virol.2005.05.011 ER -