Syndecan-4 Is Expressed by B Lineage Lymphocytes and Can Transmit a Signal for Formation of Dendritic Processes Export

@article{citeulike:5972787, abstract = {Our previous studies indicated that stromal cell-derived syndecan-4 might mediate some form of communication with pre-B cells in bone marrow. We now report additional aspects of this recognition and show that syndecan-4 is also present on pre-B cells. Indeed, the molecule is acquired at an early stage of differentiation and retained until mature B cells undergo Ig isotype switching. mAbs developed to two portions of the syndecan-4 protein core were used to probe possible functions on B lineage lymphocytes. Syndecan-4 ligation had no obvious influence on B lymphocyte formation or activation, but this treatment caused a dramatic morphological change in appropriately stimulated leukocytes. Extended filopodia appeared on transfected Ba/F3 or FDCP-1 cells, as well as activated B cell blasts that were placed on syndecan-4 Ab-coated surfaces. The dendritic processes contained polymerized actin as well as pp52(LSP1), a prominent F-actin binding protein in lymphocytes. The cytoplasmic domain of syndecan-4 was not required for this response. Shape changes of this type could facilitate interactions between B lymphocytes and other components of the immune system. Not only is syndecan-4 a useful marker for discriminating normal B lineage lymphocyte subsets, but our results suggest new ways for the syndecans to participate in immune responses.}, author = {Yamashita, Yoshio and Oritani, Kenji and Miyoshi, Erina K. and Wall, Randolph and Bernfield, Merton and Kincade, Paul W.}, citeulike-article-id = {5972787}, citeulike-linkout-0 = {http://www.jimmunol.org/content/162/10/5940.abstract}, citeulike-linkout-1 = {http://www.jimmunol.org/content/162/10/5940.full.pdf}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/10229831}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=10229831}, comment = {10.14.09}, day = {15}, issn = {0022-1767}, journal = {J Immunol}, keywords = {b, cell, syndecan}, month = {May}, number = {10}, pages = {5940--5948}, posted-at = {2009-10-20 01:11:23}, priority = {0}, title = {Syndecan-4 Is Expressed by B Lineage Lymphocytes and Can Transmit a Signal for Formation of Dendritic Processes}, url = {http://www.jimmunol.org/content/162/10/5940.abstract}, volume = {162}, year = {1999} }

TY - JOUR ID - citeulike:5972787 L3 - citeulike-article-id:5972787 N2 - Our previous studies indicated that stromal cell-derived syndecan-4 might mediate some form of communication with pre-B cells in bone marrow. We now report additional aspects of this recognition and show that syndecan-4 is also present on pre-B cells. Indeed, the molecule is acquired at an early stage of differentiation and retained until mature B cells undergo Ig isotype switching. mAbs developed to two portions of the syndecan-4 protein core were used to probe possible functions on B lineage lymphocytes. Syndecan-4 ligation had no obvious influence on B lymphocyte formation or activation, but this treatment caused a dramatic morphological change in appropriately stimulated leukocytes. Extended filopodia appeared on transfected Ba/F3 or FDCP-1 cells, as well as activated B cell blasts that were placed on syndecan-4 Ab-coated surfaces. The dendritic processes contained polymerized actin as well as pp52(LSP1), a prominent F-actin binding protein in lymphocytes. The cytoplasmic domain of syndecan-4 was not required for this response. Shape changes of this type could facilitate interactions between B lymphocytes and other components of the immune system. Not only is syndecan-4 a useful marker for discriminating normal B lineage lymphocyte subsets, but our results suggest new ways for the syndecans to participate in immune responses. IS - 10 JF - J Immunol SN - 0022-1767 EP - 5948 TI - Syndecan-4 Is Expressed by B Lineage Lymphocytes and Can Transmit a Signal for Formation of Dendritic Processes VL - 162 SP - 5940 KW - b KW - cell KW - syndecan N1 - 10.14.09 AU - Yamashita, Yoshio AU - Oritani, Kenji AU - Miyoshi, Erina AU - Wall, Randolph AU - Bernfield, Merton AU - Kincade, Paul PY - 1999/05/15/ UR - http://www.jimmunol.org/content/162/10/5940.abstract ER -