Selective activation of TACI by syndecan-2 Export

@article{citeulike:5983356, abstract = {B-lymphocyte homeostasis and function are regulated by complementary actions of the TNFR family members TACI, BCMA, and BAFF-R, which are expressed by mature B cells. How these receptors are differentially activated is not entirely understood, because the primary ligand BAFF binds to all three. We searched for alternative ligands for TACI using recombinant TACI-Fc fusion protein as a probe and identified syndecan-2 as a new binding partner. TACI binding appears to require heparan sulfate posttranslational modifications of syndecan-2, because free heparin or pretreatment with heparitinase blocked the interaction. Syndecan-2 bound TACI but bound neither BAFF-R nor BCMA. Transfected cells expressing syndecan-2 activated signaling through TACI, as indicated by an NFAT-specific reporter. Syndecan-1 and syndecan-4 were also able to induce TACI signaling in a similar manner. This is the first identification of ligands that selectively activate TACI without simultaneously triggering BCMA or BAFF-R. This finding may help explain the alternative outcomes of signaling from this family of receptors in B cells. 10.1182/blood-2005-01-0256}, author = {Bischof, Daniela and Elsawa, Sherine F. and Mantchev, George and Yoon, Juhan and Michels, Grace E. and Nilson, Allan and Sutor, Shari L. and Platt, Jeffrey L. and Ansell, Stephen M. and von Bulow, Gotz and Bram, Richard J.}, citeulike-article-id = {5983356}, citeulike-linkout-0 = {http://dx.doi.org/10.1182/blood-2005-01-0256}, citeulike-linkout-1 = {http://bloodjournal.hematologylibrary.org/content/107/8/3235.abstract}, citeulike-linkout-2 = {http://bloodjournal.hematologylibrary.org/content/107/8/3235.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/16357320}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=16357320}, comment = {11.3.09. erroneous? }, day = {15}, doi = {10.1182/blood-2005-01-0256}, journal = {Blood}, keywords = {april, hspg, taci}, month = {April}, number = {8}, pages = {3235--3242}, posted-at = {2009-10-22 01:48:25}, priority = {0}, title = {Selective activation of TACI by syndecan-2}, url = {http://dx.doi.org/10.1182/blood-2005-01-0256}, volume = {107}, year = {2006} }

TY - JOUR ID - citeulike:5983356 L3 - citeulike-article-id:5983356 N2 - B-lymphocyte homeostasis and function are regulated by complementary actions of the TNFR family members TACI, BCMA, and BAFF-R, which are expressed by mature B cells. How these receptors are differentially activated is not entirely understood, because the primary ligand BAFF binds to all three. We searched for alternative ligands for TACI using recombinant TACI-Fc fusion protein as a probe and identified syndecan-2 as a new binding partner. TACI binding appears to require heparan sulfate posttranslational modifications of syndecan-2, because free heparin or pretreatment with heparitinase blocked the interaction. Syndecan-2 bound TACI but bound neither BAFF-R nor BCMA. Transfected cells expressing syndecan-2 activated signaling through TACI, as indicated by an NFAT-specific reporter. Syndecan-1 and syndecan-4 were also able to induce TACI signaling in a similar manner. This is the first identification of ligands that selectively activate TACI without simultaneously triggering BCMA or BAFF-R. This finding may help explain the alternative outcomes of signaling from this family of receptors in B cells. 10.1182/blood-2005-01-0256 IS - 8 JF - Blood EP - 3242 TI - Selective activation of TACI by syndecan-2 VL - 107 SP - 3235 KW - april KW - hspg KW - taci N1 - 11.3.09. erroneous? AU - Bischof, Daniela AU - Elsawa, Sherine AU - Mantchev, George AU - Yoon, Juhan AU - Michels, Grace AU - Nilson, Allan AU - Sutor, Shari AU - Platt, Jeffrey AU - Ansell, Stephen AU - von Bulow, Gotz AU - Bram, Richard PY - 2006/04/15/ UR - http://dx.doi.org/10.1182/blood-2005-01-0256 ER -