Cytoskeletal dynamics of human erythrocyte Export

@article{citeulike:1457110, abstract = {10.1073/pnas.0700257104 The human erythrocyte (red blood cell, RBC) demonstrates extraordinary ability to undergo reversible large deformation and fluidity. Such mechanical response cannot be consistently rationalized on the basis of fixed connectivity of the cell cytoskeleton that comprises the spectrin molecular network tethered to phospholipid membrane. Active topological remodeling of spectrin network has been postulated, although detailed models of such dynamic reorganization are presently unavailable. Here we present a coarse-grained cytoskeletal dynamics simulation with breakable protein associations to elucidate the roles of shear stress, specific chemical agents, and thermal fluctuations in cytoskeleton remodeling. We demonstrate a clear solid-to-fluid transition depending on the metabolic energy influx. The solid network's plastic deformation also manifests creep and yield regimes depending on the strain rate. This cytoskeletal dynamics model offers a means to resolve long-standing questions regarding the reference state used in RBC elasticity theory for determining the equilibrium shape and deformation response. In addition, the simulations offer mechanistic insights into the onset of plasticity and void percolation in cytoskeleton. These phenomena may have implication for RBC membrane loss and shape change in the context of hereditary hemolytic disorders such as spherocytosis and elliptocytosis.}, address = {Department of Materials Science and Engineering, Ohio State University, Columbus, OH 43210, USA.}, author = {Li, Ju and Lykotrafitis, George and Dao, Ming and Suresh, Subra}, citeulike-article-id = {1457110}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0700257104}, citeulike-linkout-1 = {http://www.pnas.org/content/104/12/4937.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/104/12/4937.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/17360346}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=17360346}, day = {20}, doi = {10.1073/pnas.0700257104}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences}, keywords = {blood, cell, cytoskeletal, erythrocyte, rbc, red, structure}, month = {March}, number = {12}, pages = {4937--4942}, posted-at = {2009-11-02 14:52:34}, priority = {2}, title = {Cytoskeletal dynamics of human erythrocyte}, url = {http://dx.doi.org/10.1073/pnas.0700257104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:1457110 L3 - citeulike-article-id:1457110 N2 - 10.1073/pnas.0700257104 The human erythrocyte (red blood cell, RBC) demonstrates extraordinary ability to undergo reversible large deformation and fluidity. Such mechanical response cannot be consistently rationalized on the basis of fixed connectivity of the cell cytoskeleton that comprises the spectrin molecular network tethered to phospholipid membrane. Active topological remodeling of spectrin network has been postulated, although detailed models of such dynamic reorganization are presently unavailable. Here we present a coarse-grained cytoskeletal dynamics simulation with breakable protein associations to elucidate the roles of shear stress, specific chemical agents, and thermal fluctuations in cytoskeleton remodeling. We demonstrate a clear solid-to-fluid transition depending on the metabolic energy influx. The solid network's plastic deformation also manifests creep and yield regimes depending on the strain rate. This cytoskeletal dynamics model offers a means to resolve long-standing questions regarding the reference state used in RBC elasticity theory for determining the equilibrium shape and deformation response. In addition, the simulations offer mechanistic insights into the onset of plasticity and void percolation in cytoskeleton. These phenomena may have implication for RBC membrane loss and shape change in the context of hereditary hemolytic disorders such as spherocytosis and elliptocytosis. IS - 12 JF - Proceedings of the National Academy of Sciences SN - 0027-8424 AD - Department of Materials Science and Engineering, Ohio State University, Columbus, OH 43210, USA. EP - 4942 TI - Cytoskeletal dynamics of human erythrocyte VL - 104 SP - 4937 KW - blood KW - cell KW - cytoskeletal KW - erythrocyte KW - rbc KW - red KW - structure AU - Li, Ju AU - Lykotrafitis, George AU - Dao, Ming AU - Suresh, Subra PY - 2007/03/20/ UR - http://dx.doi.org/10.1073/pnas.0700257104 ER -