Comparison of the Results of SDS PAGE and Chip Electrophoresis of Wheat Storage Proteins Export

@article{citeulike:5378320, abstract = {Abstract\ \ Chip electrophoresis was used for the study of the high-molecular-weight glutenin subunit (HMW-GS) composition of wheat. A model set of samples of Czech wheat varieties was used and HMW-GSs were detected by SDS-PAGE. A part of the common HMW-GSs present in wheat were identified by chip electrophoresis. Chip electrophoresis provides a very good reproducibility of HMW-GS patterns. Moreover this approach is much faster than the conventional SDS PAGE methods requiring several hours for an analysis. Another advantage over traditional gel electrophoresis is lower sample and reagent volume requirements, as well as specialized protein standards for accurate reproducibility and quantification.}, author = {Bradov\'{a}, Jana and Mat\v{e}jov\'{a}, Eva}, citeulike-article-id = {5378320}, citeulike-linkout-0 = {http://dx.doi.org/10.1365/s10337-008-0545-2}, citeulike-linkout-1 = {http://www.springerlink.com/content/j519250661671421}, day = {1}, doi = {10.1365/s10337-008-0545-2}, journal = {Chromatographia}, keywords = {chip, electrophoresis, proteins, sds-page, wheat}, month = {May}, number = {0}, pages = {83--88}, posted-at = {2009-11-10 09:51:18}, priority = {2}, title = {Comparison of the Results of SDS PAGE and Chip Electrophoresis of Wheat Storage Proteins}, url = {http://dx.doi.org/10.1365/s10337-008-0545-2}, volume = {67}, year = {2008} }

TY - JOUR ID - citeulike:5378320 L3 - citeulike-article-id:5378320 N2 - Abstract  Chip electrophoresis was used for the study of the high-molecular-weight glutenin subunit (HMW-GS) composition of wheat. A model set of samples of Czech wheat varieties was used and HMW-GSs were detected by SDS-PAGE. A part of the common HMW-GSs present in wheat were identified by chip electrophoresis. Chip electrophoresis provides a very good reproducibility of HMW-GS patterns. Moreover this approach is much faster than the conventional SDS PAGE methods requiring several hours for an analysis. Another advantage over traditional gel electrophoresis is lower sample and reagent volume requirements, as well as specialized protein standards for accurate reproducibility and quantification. IS - 0 JF - Chromatographia EP - 88 TI - Comparison of the Results of SDS PAGE and Chip Electrophoresis of Wheat Storage Proteins VL - 67 SP - 83 KW - chip KW - electrophoresis KW - proteins KW - sds-page KW - wheat AU - Bradová, Jana AU - Matějová, Eva PY - 2008/05/01/ UR - http://dx.doi.org/10.1365/s10337-008-0545-2 ER -