Genomic inferences of the cis-regulatory nucleotide polymorphisms underlying gene expression differences between Drosophila melanogaster mating races. Export

@article{citeulike:691433, abstract = {Nucleotide sequence polymorphisms affecting gene expression occur in the regulatory region of genes (in cis) and elsewhere in the genome (in trans). Further study is required to weigh the relative importance of cis- and trans-acting mutations in mediating gene expression differences within and between species. Here, microarray hybridization experiments were used to isolate 363 gene expression differences between the female fly head transcriptomes of 2 Drosophila melanogaster strains. One strain (French) represented the cosmopolitan M mating race and the other strain (ZS30) represented the Z mating race derived from Zimbabwe, Africa. From chromosomal substitution strains engineered from the 2 strains, we inferred that the expression differences between M and Z alleles largely could be attributed to the genotype of the chromosomes where the differentially expressed genes were located, that is, cis-regulatory polymorphisms prominently influence gene expression differences between M and Z. The effects of trans-regulatory polymorphisms were apparent yet difficult to quantify. Results have implications for models of gene regulatory evolution as well as experimental studies trying to identify the nucleotide sequence polymorphisms underlying gene expression differences between Drosophila strains.}, address = {Division of Biomedical Research Resources, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.}, author = {Osada, Naoki and Kohn, Michael H. and Wu, Chung-I I.}, citeulike-article-id = {691433}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/molbev/msl023}, citeulike-linkout-1 = {http://mbe.oxfordjournals.org/cgi/content/abstract/23/8/1585}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16754642}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16754642}, day = {5}, doi = {10.1093/molbev/msl023}, issn = {0737-4038}, journal = {Molecular biology and evolution}, keywords = {cis\_vs\_trans, drosophila, expression\_evolution, population\_genetics}, month = {August}, number = {8}, pages = {1585--1591}, posted-at = {2009-11-06 15:47:52}, priority = {2}, title = {Genomic inferences of the cis-regulatory nucleotide polymorphisms underlying gene expression differences between Drosophila melanogaster mating races.}, url = {http://dx.doi.org/10.1093/molbev/msl023}, volume = {23}, year = {2006} }

TY - JOUR ID - citeulike:691433 L3 - citeulike-article-id:691433 N2 - Nucleotide sequence polymorphisms affecting gene expression occur in the regulatory region of genes (in cis) and elsewhere in the genome (in trans). Further study is required to weigh the relative importance of cis- and trans-acting mutations in mediating gene expression differences within and between species. Here, microarray hybridization experiments were used to isolate 363 gene expression differences between the female fly head transcriptomes of 2 Drosophila melanogaster strains. One strain (French) represented the cosmopolitan M mating race and the other strain (ZS30) represented the Z mating race derived from Zimbabwe, Africa. From chromosomal substitution strains engineered from the 2 strains, we inferred that the expression differences between M and Z alleles largely could be attributed to the genotype of the chromosomes where the differentially expressed genes were located, that is, cis-regulatory polymorphisms prominently influence gene expression differences between M and Z. The effects of trans-regulatory polymorphisms were apparent yet difficult to quantify. Results have implications for models of gene regulatory evolution as well as experimental studies trying to identify the nucleotide sequence polymorphisms underlying gene expression differences between Drosophila strains. IS - 8 JF - Molecular biology and evolution SN - 0737-4038 AD - Division of Biomedical Research Resources, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan. EP - 1591 TI - Genomic inferences of the cis-regulatory nucleotide polymorphisms underlying gene expression differences between Drosophila melanogaster mating races. VL - 23 SP - 1585 KW - cis_vs_trans KW - drosophila KW - expression_evolution KW - population_genetics AU - Osada, Naoki AU - Kohn, Michael AU - Wu, Chung-I PY - 2006/08/05/ UR - http://dx.doi.org/10.1093/molbev/msl023 ER -