Rapid method for the characterization of 3' and 5' UTRs of influenza viruses. Export

@article{citeulike:1193339, abstract = {A T4 RNA ligase based strategy is demonstrated that allows for the full characterization of 3' and 5' UTR regions of negative strand RNA viruses. Negative strand RNA viruses such as influenza have 3'OH and 5'P terminal ends that are capable of being ligated using T4 RNA ligase. Each segment can form a mixture of linear concatamers between like and different viral segments or can itself form a circular structure upon ligation. RT-PCR can then be performed on these circular RNA segments using gene specific primers subsequently allowing for the characterization of the true terminal sequence for each viral segment. The UTR regions of a number of influenza virus strains were defined accurately using this approach.}, address = {Department of Virus and Cell Biology, Merck and Co Inc, 44L-206B West Point, PA 19486, USA.}, author = {Szymkowiak, C. and Kwan, W. S. and Su, Q. and Toner, T. J. and Shaw, A. R. and Youil, R.}, citeulike-article-id = {1193339}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12445933}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12445933}, issn = {0166-0934}, journal = {J Virol Methods}, keywords = {influenza-virus, molecular\_methods}, month = {January}, number = {1}, pages = {15--20}, posted-at = {2007-03-28 20:56:45}, priority = {2}, title = {Rapid method for the characterization of 3' and 5' UTRs of influenza viruses.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12445933}, volume = {107}, year = {2003} }

TY - JOUR ID - citeulike:1193339 L3 - citeulike-article-id:1193339 N2 - A T4 RNA ligase based strategy is demonstrated that allows for the full characterization of 3' and 5' UTR regions of negative strand RNA viruses. Negative strand RNA viruses such as influenza have 3'OH and 5'P terminal ends that are capable of being ligated using T4 RNA ligase. Each segment can form a mixture of linear concatamers between like and different viral segments or can itself form a circular structure upon ligation. RT-PCR can then be performed on these circular RNA segments using gene specific primers subsequently allowing for the characterization of the true terminal sequence for each viral segment. The UTR regions of a number of influenza virus strains were defined accurately using this approach. IS - 1 JF - J Virol Methods SN - 0166-0934 AD - Department of Virus and Cell Biology, Merck and Co Inc, 44L-206B West Point, PA 19486, USA. EP - 20 TI - Rapid method for the characterization of 3' and 5' UTRs of influenza viruses. VL - 107 SP - 15 KW - influenza-virus KW - molecular_methods AU - Szymkowiak, C AU - Kwan, WS AU - Su, Q AU - Toner, TJ AU - Shaw, AR AU - Youil, R PY - 2003/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/12445933 ER -