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New Cloning Vectors with Temperature-Sensitive Replication

by: Gregory J Phillips
Plasmid, Vol. 41, No. 1. (January 1999), pp. 78-81.

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A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restriction sites and provide for screening of recombinant plasmids by [alpha] complementation. These vectors have proven useful for a variety of applications where conditional replication of a recombinant plasmid is desired.


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