Design Parameters to Control Synthetic Gene Expression in Escherichia coli Export

@article{citeulike:5781713, abstract = {Production of proteins as therapeutic agents, research reagents and molecular tools frequently depends on expression in heterologous hosts. Synthetic genes are increasingly used for protein production because sequence information is easier to obtain than the corresponding physical DNA. Protein-coding sequences are commonly re-designed to enhance expression, but there are no experimentally supported design principles.}, author = {Welch, Mark and Govindarajan, Sridhar and Ness, Jon E. and Villalobos, Alan and Gurney, Austin and Minshull, Jeremy and Gustafsson, Claes}, citeulike-article-id = {5781713}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pone.0007002}, day = {14}, doi = {10.1371/journal.pone.0007002}, journal = {PLoS ONE}, keywords = {gene\_expression, genetic\_code, synthetic\_biology, synthetic\_dna, synthetic\_gene}, month = {September}, number = {9}, pages = {e7002+}, posted-at = {2009-11-05 14:39:25}, priority = {2}, publisher = {Public Library of Science}, title = {Design Parameters to Control Synthetic Gene Expression in Escherichia coli}, url = {http://dx.doi.org/10.1371/journal.pone.0007002}, volume = {4}, year = {2009} }

TY - JOUR ID - citeulike:5781713 L3 - citeulike-article-id:5781713 N2 - Production of proteins as therapeutic agents, research reagents and molecular tools frequently depends on expression in heterologous hosts. Synthetic genes are increasingly used for protein production because sequence information is easier to obtain than the corresponding physical DNA. Protein-coding sequences are commonly re-designed to enhance expression, but there are no experimentally supported design principles. IS - 9 JF - PLoS ONE TI - Design Parameters to Control Synthetic Gene Expression in Escherichia coli PB - Public Library of Science VL - 4 SP - e7002 KW - gene_expression KW - genetic_code KW - synthetic_biology KW - synthetic_dna KW - synthetic_gene AU - Welch, Mark AU - Govindarajan, Sridhar AU - Ness, Jon AU - Villalobos, Alan AU - Gurney, Austin AU - Minshull, Jeremy AU - Gustafsson, Claes PY - 2009/09/14/ UR - http://dx.doi.org/10.1371/journal.pone.0007002 ER -