Reactive polymer coatings: a first step toward surface engineering of microfluidic devices. Export

@article{citeulike:6032308, abstract = {We report fabrication, characterization, and use of microfluidic analysis devices containing surface-immobilized cell-capturing molecules. Amino-terminated biotin ligands are immobilized onto the luminal surface of a microdevice and effectively support self-assembly of proteins, antibodies, and mammalian cells. For this purpose, chemical vapor deposition (CVD) polymerization is used to functionalize PDMS-made microfluidic devices with poly[para-xylylene carboxylic acid pentafluorophenolester-co-para-xylylene]. The resulting reactive coating shows excellent adhesion when deposited in thin films (approximately 100 nm, and the distribution of the pentafluorophenol ester groups is reasonably uniform within the microchannel inner surface, as examined by fluorescence microscopy. The utility of these devices for cell-based bioassays is demonstrated by monitoring the concentration-dependent effect of the disintegrin echistatin on cell adhesion. The described assay format could be relevant to clinical research in various fields, including angiogenesis research.}, author = {Lahann, J\"{o}rg and Balcells, Mercedes and Lu, Hang and Rodon, Teresa and Jensen, Klavs F. and Langer, Robert}, citeulike-article-id = {6032308}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ac020557s}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ac020557s}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12720350}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12720350}, day = {1}, doi = {10.1021/ac020557s}, issn = {0003-2700}, journal = {Analytical chemistry}, month = {May}, number = {9}, pages = {2117--2122}, posted-at = {2009-10-29 14:33:43}, priority = {2}, title = {Reactive polymer coatings: a first step toward surface engineering of microfluidic devices.}, url = {http://dx.doi.org/10.1021/ac020557s}, volume = {75}, year = {2003} }

TY - JOUR ID - citeulike:6032308 L3 - citeulike-article-id:6032308 N2 - We report fabrication, characterization, and use of microfluidic analysis devices containing surface-immobilized cell-capturing molecules. Amino-terminated biotin ligands are immobilized onto the luminal surface of a microdevice and effectively support self-assembly of proteins, antibodies, and mammalian cells. For this purpose, chemical vapor deposition (CVD) polymerization is used to functionalize PDMS-made microfluidic devices with poly[para-xylylene carboxylic acid pentafluorophenolester-co-para-xylylene]. The resulting reactive coating shows excellent adhesion when deposited in thin films (approximately 100 nm, and the distribution of the pentafluorophenol ester groups is reasonably uniform within the microchannel inner surface, as examined by fluorescence microscopy. The utility of these devices for cell-based bioassays is demonstrated by monitoring the concentration-dependent effect of the disintegrin echistatin on cell adhesion. The described assay format could be relevant to clinical research in various fields, including angiogenesis research. IS - 9 JF - Analytical chemistry SN - 0003-2700 EP - 2122 TI - Reactive polymer coatings: a first step toward surface engineering of microfluidic devices. VL - 75 SP - 2117 AU - Lahann, Jörg AU - Balcells, Mercedes AU - Lu, Hang AU - Rodon, Teresa AU - Jensen, Klavs AU - Langer, Robert PY - 2003/05/01/ UR - http://dx.doi.org/10.1021/ac020557s ER -