Effects of Native Human Zona Pellucida Glycoproteins 3 and 4 on Acrosome Reaction and Zona Pellucida Binding of Human Spermatozoa Export

@article{citeulike:3181284, abstract = {Acrosome reaction is crucial to the penetration of spermatozoa through the zona pellucida (ZP). Glycosylation of ZP glycoproteins is important in spermatozoa-ZP interaction. Human ZP glycoprotein-3 (ZP3) is believed to initiate acrosome reaction. Recently, human ZP4 is also implicated in inducing acrosome reaction. These studies are based on recombinant human ZP proteins with glycosylation different from their native counterparts. In the present study, the effects of native human ZP3 and ZP4 on acrosome reaction and spermatozoa-ZP binding were investigated. Native human ZP3 and ZP4 were immunoaffinity-purified. They induced acrosome reaction and inhibited spermatozoa-ZP binding time- and dose-dependently to different extents. These biological activities of human ZP3 and ZP4 depended partly on their glycosylation with N-linked glycosylation contributed much more significantly than O-linked glycosylation. Studies with inhibitors showed that both human ZP3- and ZP4-induced acrosome reaction were protein kinase-C, protein tyrosine kinase, T-type Ca2+ channels and extracellular Ca2+ dependent. G-protein also participated in human ZP3-, but not in ZP4-induced acrosome reaction. On the other hand, protein kinase-A and L-type Ca2+ channels took part only in human ZP4-induced acrosome reaction. This manuscript describes for the first time, the actions of purified native human ZP3 and ZP4 on acrosome reaction and spermatozoa-ZP binding. 10.1095/biolreprod.108.069344}, author = {Chiu, Philip C. and Wong, Ben S. and Chung, Man-Kin and Lam, Kevin K. and Pang, Ronald T. and Lee, Kai-Fai and Sumitro, S. B. and Gupta, S. K. and Yeung, William S.}, citeulike-article-id = {3181284}, citeulike-linkout-0 = {http://dx.doi.org/10.1095/biolreprod.108.069344}, citeulike-linkout-1 = {http://www.biolreprod.org/cgi/content/abstract/79/5/869}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18667750}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18667750}, day = {30}, doi = {10.1095/biolreprod.108.069344}, journal = {Biol Reprod}, keywords = {acrosome-reaction, zona-pellucida}, month = {July}, pages = {biolreprod.108.069344+}, posted-at = {2008-09-02 11:07:09}, priority = {2}, title = {Effects of Native Human Zona Pellucida Glycoproteins 3 and 4 on Acrosome Reaction and Zona Pellucida Binding of Human Spermatozoa}, url = {http://dx.doi.org/10.1095/biolreprod.108.069344}, year = {2008} }

TY - JOUR ID - citeulike:3181284 L3 - citeulike-article-id:3181284 N2 - Acrosome reaction is crucial to the penetration of spermatozoa through the zona pellucida (ZP). Glycosylation of ZP glycoproteins is important in spermatozoa-ZP interaction. Human ZP glycoprotein-3 (ZP3) is believed to initiate acrosome reaction. Recently, human ZP4 is also implicated in inducing acrosome reaction. These studies are based on recombinant human ZP proteins with glycosylation different from their native counterparts. In the present study, the effects of native human ZP3 and ZP4 on acrosome reaction and spermatozoa-ZP binding were investigated. Native human ZP3 and ZP4 were immunoaffinity-purified. They induced acrosome reaction and inhibited spermatozoa-ZP binding time- and dose-dependently to different extents. These biological activities of human ZP3 and ZP4 depended partly on their glycosylation with N-linked glycosylation contributed much more significantly than O-linked glycosylation. Studies with inhibitors showed that both human ZP3- and ZP4-induced acrosome reaction were protein kinase-C, protein tyrosine kinase, T-type Ca2+ channels and extracellular Ca2+ dependent. G-protein also participated in human ZP3-, but not in ZP4-induced acrosome reaction. On the other hand, protein kinase-A and L-type Ca2+ channels took part only in human ZP4-induced acrosome reaction. This manuscript describes for the first time, the actions of purified native human ZP3 and ZP4 on acrosome reaction and spermatozoa-ZP binding. 10.1095/biolreprod.108.069344 JF - Biol Reprod TI - Effects of Native Human Zona Pellucida Glycoproteins 3 and 4 on Acrosome Reaction and Zona Pellucida Binding of Human Spermatozoa SP - biolreprod.108.069344 KW - acrosome-reaction KW - zona-pellucida AU - Chiu, Philip AU - Wong, Ben AU - Chung, Man-Kin AU - Lam, Kevin AU - Pang, Ronald AU - Lee, Kai-Fai AU - Sumitro, SB AU - Gupta, SK AU - Yeung, William PY - 2008/07/30/ UR - http://dx.doi.org/10.1095/biolreprod.108.069344 ER -